Document Detail

Galactose-functionalized magnetic iron-oxide nanoparticles for enrichment and detection of ricin toxin.
MedLine Citation:
PMID:  21233555     Owner:  NLM     Status:  In-Process    
To generate a new specific recognition module for the enrichment and detection of ricin, galactose-functionalized silanized magnetic iron-oxide nanoparticles (Gal-SiMNPs) were assembled by amino-silanized MNPs (amino-SiMNPs) and a galactose linker. Amino-SiMNPs were produced by a coprecipitation method, and were coated with double layers of silica and aminosilane by hydrolysis of tetraethoxysilane and 3-aminopropyltriethoxysilane, respectively. A galactose with an amido-acid linker was synthesized by four steps of chemical modification from O-acetyl protected galactose. The diameters of Gal-SiMNPs were characterized as being 60 ± 20 nm. The average amount of galactose-loading and ricin-binding on Gal-SiMNPs was 30 ± 2 µg galactose and 29 ± 2 µg ricin toxin on the surface of 1 mg of Gal-SiMNPs, respectively. Furthermore, a rapid, simple and efficient colorimetric assay was established for the detection of ricin based on the Gal-SiMNPs, and the limits of detection (LODs) of 2 and 4 ng/mL for ricin in physiological buffer and serum were obtained, respectively.
He-Zhu Liu; Ji-Jun Tang; Xiao-Xi Ma; Lei Guo; Jian-Wei Xie; Yu-Xia Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  27     ISSN:  1348-2246     ISO Abbreviation:  Anal Sci     Publication Date:  2011  
Date Detail:
Created Date:  2011-01-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  19-24     Citation Subset:  IM    
Beijing Institute of Pharmacology and Toxicology, No.27 Tai Ping Road, Hai Dian District, Beijing 100850, P. R. China.
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