| Galactose-binding lectins as markers of pregnancy-related glycoproteins. | |
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MedLine Citation:
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PMID: 8468198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Protein extracts from pregnant mouse endometria were compared with those obtained from non-pregnant and pseudopregnant mice to detect early pregnancy-specific galactose-rich glycoproteins. Gradient gel electrophoresis combined with lectin overlay and lectin histochemistry were used to identify Ricinus communis I (RCA-I), R. communis II (RCA-II) and Cytisus scoparius (CSA) lectin binding glycoproteins. Using this approach, galactose-rich glycoproteins were identified that were maximally expressed in the estrus phase of non-pregnant endometria and also those that had peak expression in pregnancy. Lectin histochemistry revealed pregnancy related changes in three portions of mouse endometrium: endometrial glands, luminal epithelium and its basement membrane. Two major glycoproteins (RCA-I reactive 64 kDa and RCA-II reactive 35 kDa) were specifically expressed in peri-implantation endometrium on days 3 and 4 of pregnancy. The appearance of these glycoproteins during the period of the implantation window in mouse suggests that they could serve as markers of uterine receptivity to the implanting blastocyst. |
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Authors:
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B Horvat |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Histochemistry Volume: 99 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1993 Jan |
Date Detail:
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Created Date: 1993-05-10 Completed Date: 1993-05-10 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY |
Other Details:
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Languages: eng Pagination: 95-101 Citation Subset: IM |
Affiliation:
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Department of Pathology and Cell Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism Animals Binding Sites Biological Markers Electrophoresis, Polyacrylamide Gel Endometrium / chemistry Estrus / physiology Female Galactose / metabolism* Glycoproteins / metabolism* Histocytochemistry Lectins / diagnostic use* Mice Pregnancy Pregnancy, Animal / metabolism* Ricin Uterus / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Glycoproteins; 0/Lectins; 26566-61-0/Galactose; 9009-86-3/Ricin; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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