Document Detail


Galactolipid formation in chloroplast envelopes. II. Isolation-induced changes in galactolipid composition.
MedLine Citation:
PMID:  7213774     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Spinach envelopes were isolated at pH 8.5, which is inhibitory to interlipid galactosyl transfer, and at pH 7.2, which is close to values used in most recent work on chloroplast envelopes. Peptide patterns were identical, but galactolipid compositions differed markedly. These changes are such as could be explained by interlipid galactosyl transfer during isolation at pH 7.2, i.e. a strong decrease in monogalactolipid and a corresponding increase in tri- and tetragalactolipids and diacylglycerol. Digalactolipid was little affected. It was further observed that the interlipid galactosyl transfer was restricted to lipids rich in hexadecatrienoic acid. Arguments are presented that the lipid composition of envelopes in vivo is better represented by the results obtained at high pH. The results will be discussed in relation to previous data on galactolipid composition and biosynthesis in envelopes.
Authors:
J F Wintermans; A Van Besouw; G Bögemann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  663     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-06-25     Completed Date:  1981-06-25     Revised Date:  2002-11-01    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  99-107     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chloroplasts / analysis*
Chromatography, Thin Layer
Fatty Acids / analysis
Galactolipids
Glycolipids / isolation & purification*
Intracellular Membranes / analysis*
Membrane Lipids / isolation & purification*
Plants / analysis
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Galactolipids; 0/Glycolipids; 0/Membrane Lipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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