Document Detail


Galactolipid Synthesis in Vicia faba Leaves: IV. Site(s) of Fatty Acid Incorporation into the Major Glycerolipids.
MedLine Citation:
PMID:  16660788     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The fatty acids of the major glycerolipids of Vicia faba leaves were analyzed immediately following (14)CO(2) feeding. The leaves were fractionated into chloroplast and cytoplasmic fractions and the location of radioactivity in the fatty acids determined. The results indicate that the major site of incorporation of fatty acids is in the phospholipids. Phosphatidylcholine contained the highest level of radioactivity in the cytoplasmic fraction, whereas phosphatidylglycerol contained radioactivity in both the chloroplast and cytoplasmic fractions. The galactolipids contained very little radioactivity in comparison, this radioactivity being confined to high speed centrifugal fractions believed to contain the envelopes of the chloroplast. Our results suggest that phosphatidylcholine is a major site of incorporation of fatty acids (mainly in oleic acid) in the cytoplasm, whereas phosphatidylglycerol is also a site of incorporation involving both oleic and palmitic acids, inside and outside the chloroplast.
Authors:
E E Simpson; J P Williams
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  63     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1979 Apr 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  674-6     Citation Subset:  -    
Affiliation:
Department of Botany, University of Toronto, Ontario M5S 1A1 Canada.
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