Document Detail


Galactoglucomannans increase cell population density and alter the protoxylem/metaxylem tracheary element ratio in xylogenic cultures of Zinnia.
MedLine Citation:
PMID:  16891547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Xylogenic cultures of zinnia (Zinnia elegans) provide a unique opportunity to study signaling pathways of tracheary element (TE) differentiation. In vitro TEs differentiate into either protoxylem (PX)-like TEs characterized by annular/helical secondary wall thickening or metaxylem (MX)-like TEs with reticulate/scalariform/pitted thickening. The factors that determine these different cell fates are largely unknown. We show here that supplementing zinnia cultures with exogenous galactoglucomannan oligosaccharides (GGMOs) derived from spruce (Picea abies) xylem had two major effects: an increase in cell population density and a decrease in the ratio of PX to MX TEs. In an attempt to link these two effects, the consequence of the plane of cell division on PX-MX differentiation was assessed. Although GGMOs did not affect the plane of cell division per se, they significantly increased the proportion of longitudinally divided cells differentiating into MX. To test the biological significance of these findings, we have determined the presence of mannan-containing oligosaccharides in zinnia cultures in vitro. Immunoblot assays indicated that beta-1,4-mannosyl epitopes accumulate specifically in TE-inductive media. These epitopes were homogeneously distributed within the thickened secondary walls of TEs when the primary cell wall was weakly labeled. Using polysaccharide analysis carbohydrate gel electrophoresis, glucomannans were specifically detected in cell walls of differentiating zinnia cultures. Finally, zinnia macroarrays probed with cDNAs from cells cultured in the presence or absence of GGMOs indicated that significantly more genes were down-regulated rather than up-regulated by GGMOs. This study constitutes a major step in the elucidation of signaling mechanisms of PX- and MX-specific genetic programs in zinnia.
Authors:
Anna Benová-Kákosová; Catherine Digonnet; Florence Goubet; Philippe Ranocha; Alain Jauneau; Edouard Pesquet; Odile Barbier; Zhinong Zhang; Peter Capek; Paul Dupree; Desana Lisková; Deborah Goffner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-04
Journal Detail:
Title:  Plant physiology     Volume:  142     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-11     Completed Date:  2007-01-19     Revised Date:  2014-10-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  696-709     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ336881;  DQ336882;  DQ336883;  DQ336884;  DQ336885;  DQ336886;  DQ336887;  DQ336888;  DQ336889;  DQ336890;  DQ336891
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MeSH Terms
Descriptor/Qualifier:
Asteraceae / cytology,  drug effects,  genetics,  metabolism*
Cell Division
Culture Media / chemistry
Gene Expression Profiling
Gene Expression Regulation, Plant
Mannans / pharmacology*
Molecular Sequence Data
Tissue Culture Techniques
Xylem / drug effects,  growth & development*,  metabolism
Grant Support
ID/Acronym/Agency:
D19624//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Culture Media; 0/Mannans
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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