Document Detail


Galacto-oligosaccharides have prebiotic activity in a dynamic in vitro colon model using a (13)C-labeling technique.
MedLine Citation:
PMID:  22623395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Galacto-oligosaccharides (GOS) are considered to be prebiotic, although the contribution of specific members of the microbiota to GOS fermentation and the exact microbial metabolites that are produced upon GOS fermentation are largely unknown. We aimed to determine this using uniformly (13)C-labeled GOS. The normal (control) medium and unlabeled or (13)C-labeled GOS was added to a dynamic, validated, in vitro model of the large-intestine containing an adult-type microbiota. Liquid-chromatography MS was used to measure the incorporation of (13)C label into metabolites. 16S-rRNA stable isotope probing coupled to a phylogenetic micro-array was used to determine label incorporation in microbial biomass. The primary members within the complex microbiota that were directly involved in GOS fermentation were shown to be Bifidobacterium longum, B. bifidum, B. catenulatum, Lactobacillus gasseri, and L. salivarius, in line with the prebiotic effect of GOS, although some other species incorporated (13)C label also. GOS fermentation led to an increase in acetate (+49%) and lactate (+23%) compared with the control. Total organic acid production was 8.50 and 7.52 mmol/g of carbohydrate fed for the GOS and control experiments, respectively. At the same time, the cumulative production of putrefactive metabolites (branched-chain fatty acids and ammonia) was reduced by 55%. Cross-feeding of metabolites from primary GOS fermenters to other members of the microbiota was observed. Our findings support a prebiotic role for GOS and its potential to act as a synbiotic in combination with certain probiotic strains.
Authors:
Annet J H Maathuis; Ellen G van den Heuvel; Margriet H C Schoterman; Koen Venema
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-23
Journal Detail:
Title:  The Journal of nutrition     Volume:  142     ISSN:  1541-6100     ISO Abbreviation:  J. Nutr.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-21     Completed Date:  2012-08-30     Revised Date:  2012-08-31    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1205-12     Citation Subset:  IM    
Affiliation:
TNO Earth, Environmental and Life Sciences, Research Group Pharmacokinetics and Human Studies, Zeist, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / metabolism
Ammonia / metabolism
Bifidobacterium / drug effects*,  metabolism
Carbon Isotopes / metabolism
Colon / drug effects*,  metabolism,  microbiology
Fatty Acids / biosynthesis
Fermentation
Galactose / metabolism,  pharmacology*
Lactic Acid / metabolism
Lactobacillus / drug effects*,  metabolism
Microarray Analysis
Models, Biological
Oligosaccharides / metabolism,  pharmacology*
Phylogeny
Prebiotics*
Probiotics*
RNA, Ribosomal, 16S
Staining and Labeling
Synbiotics
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Fatty Acids; 0/Oligosaccharides; 0/Prebiotics; 0/RNA, Ribosomal, 16S; 26566-61-0/Galactose; 50-21-5/Lactic Acid; 64-19-7/Acetic Acid; 7664-41-7/Ammonia

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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