| Galacto-oligosaccharides have prebiotic activity in a dynamic in vitro colon model using a (13)C-labeling technique. | |
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MedLine Citation:
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PMID: 22623395 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Galacto-oligosaccharides (GOS) are considered to be prebiotic, although the contribution of specific members of the microbiota to GOS fermentation and the exact microbial metabolites that are produced upon GOS fermentation are largely unknown. We aimed to determine this using uniformly (13)C-labeled GOS. The normal (control) medium and unlabeled or (13)C-labeled GOS was added to a dynamic, validated, in vitro model of the large-intestine containing an adult-type microbiota. Liquid-chromatography MS was used to measure the incorporation of (13)C label into metabolites. 16S-rRNA stable isotope probing coupled to a phylogenetic micro-array was used to determine label incorporation in microbial biomass. The primary members within the complex microbiota that were directly involved in GOS fermentation were shown to be Bifidobacterium longum, B. bifidum, B. catenulatum, Lactobacillus gasseri, and L. salivarius, in line with the prebiotic effect of GOS, although some other species incorporated (13)C label also. GOS fermentation led to an increase in acetate (+49%) and lactate (+23%) compared with the control. Total organic acid production was 8.50 and 7.52 mmol/g of carbohydrate fed for the GOS and control experiments, respectively. At the same time, the cumulative production of putrefactive metabolites (branched-chain fatty acids and ammonia) was reduced by 55%. Cross-feeding of metabolites from primary GOS fermenters to other members of the microbiota was observed. Our findings support a prebiotic role for GOS and its potential to act as a synbiotic in combination with certain probiotic strains. |
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Authors:
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Annet J H Maathuis; Ellen G van den Heuvel; Margriet H C Schoterman; Koen Venema |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-05-23 |
Journal Detail:
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Title: The Journal of nutrition Volume: 142 ISSN: 1541-6100 ISO Abbreviation: J. Nutr. Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-06-21 Completed Date: 2012-08-30 Revised Date: 2012-08-31 |
Medline Journal Info:
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Nlm Unique ID: 0404243 Medline TA: J Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 1205-12 Citation Subset: IM |
Affiliation:
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TNO Earth, Environmental and Life Sciences, Research Group Pharmacokinetics and Human Studies, Zeist, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid
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metabolism Ammonia / metabolism Bifidobacterium / drug effects*, metabolism Carbon Isotopes / metabolism Colon / drug effects*, metabolism, microbiology Fatty Acids / biosynthesis Fermentation Galactose / metabolism, pharmacology* Lactic Acid / metabolism Lactobacillus / drug effects*, metabolism Microarray Analysis Models, Biological Oligosaccharides / metabolism, pharmacology* Phylogeny Prebiotics* Probiotics* RNA, Ribosomal, 16S Staining and Labeling Synbiotics |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Fatty Acids; 0/Oligosaccharides; 0/Prebiotics; 0/RNA, Ribosomal, 16S; 26566-61-0/Galactose; 50-21-5/Lactic Acid; 64-19-7/Acetic Acid; 7664-41-7/Ammonia |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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