| Gadd45-alpha and Gadd45-gamma utilize p38 and JNK signaling pathways to induce cell cycle G2/M arrest in Hep-G2 hepatoma cells. | |
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MedLine Citation:
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PMID: 19048389 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Gadd45 family of proteins, which includes alpha, beta, and gamma isoforms, has recently been shown to play a role in the G2/M cell cycle checkpoint in response to DNA damage; however, the mechanisms by which Gadd45 proteins inhibit cell cycle control are not fully understood. Using immunohistochemical analysis, we found that protein expression of Gadd45gamma, but not Gadd45alpha, was down-regulated in hepatocellular carcinoma. We thus investigated possible mechanisms by which Gadd45alpha and Gadd45gamma might differentially induce G2/M arrest in the human hepatoma Hep-G2 cell line. Flow cytometric analysis revealed significant G2/M arrest in cells transfected with either Gadd45alpha or Gadd45gamma. Importantly, we found that expression of either Gadd45alpha or Gadd45gamma activated the P38 and JNK kinase pathways to induce G2/M arrest. Taken together, these findings suggest that the induction of G2/M arrest by Gadd45alpha or Gadd45gamma involves activation of two distinct signaling pathways in Hep-G2 hepatoma cell lines. |
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Authors:
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Na Zhu; Yan Shao; Lin Xu; Long Yu; Luhong Sun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-02 |
Journal Detail:
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Title: Molecular biology reports Volume: 36 ISSN: 1573-4978 ISO Abbreviation: Mol. Biol. Rep. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-21 Completed Date: 2010-02-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0403234 Medline TA: Mol Biol Rep Country: Netherlands |
Other Details:
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Languages: eng Pagination: 2075-85 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Nanjing Medical University, 210029 Nanjing, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Apoptosis Carcinoma, Hepatocellular / enzymology, metabolism*, pathology Cell Cycle Cell Cycle Proteins / metabolism* Down-Regulation Female G2 Phase / physiology Hep G2 Cells Humans Immunohistochemistry Intracellular Signaling Peptides and Proteins / metabolism* JNK Mitogen-Activated Protein Kinases / metabolism* Liver Neoplasms / enzymology, metabolism*, pathology MAP Kinase Signaling System* Male Middle Aged Nuclear Proteins / metabolism* Transfection p38 Mitogen-Activated Protein Kinases / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/GADD45A protein, human; 0/GADD45G protein, human; 0/Intracellular Signaling Peptides and Proteins; 0/Nuclear Proteins; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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