Document Detail

Gadd45-alpha and Gadd45-gamma utilize p38 and JNK signaling pathways to induce cell cycle G2/M arrest in Hep-G2 hepatoma cells.
MedLine Citation:
PMID:  19048389     Owner:  NLM     Status:  MEDLINE    
The Gadd45 family of proteins, which includes alpha, beta, and gamma isoforms, has recently been shown to play a role in the G2/M cell cycle checkpoint in response to DNA damage; however, the mechanisms by which Gadd45 proteins inhibit cell cycle control are not fully understood. Using immunohistochemical analysis, we found that protein expression of Gadd45gamma, but not Gadd45alpha, was down-regulated in hepatocellular carcinoma. We thus investigated possible mechanisms by which Gadd45alpha and Gadd45gamma might differentially induce G2/M arrest in the human hepatoma Hep-G2 cell line. Flow cytometric analysis revealed significant G2/M arrest in cells transfected with either Gadd45alpha or Gadd45gamma. Importantly, we found that expression of either Gadd45alpha or Gadd45gamma activated the P38 and JNK kinase pathways to induce G2/M arrest. Taken together, these findings suggest that the induction of G2/M arrest by Gadd45alpha or Gadd45gamma involves activation of two distinct signaling pathways in Hep-G2 hepatoma cell lines.
Na Zhu; Yan Shao; Lin Xu; Long Yu; Luhong Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-02
Journal Detail:
Title:  Molecular biology reports     Volume:  36     ISSN:  1573-4978     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-21     Completed Date:  2010-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2075-85     Citation Subset:  IM    
Department of Microbiology, Nanjing Medical University, 210029 Nanjing, People's Republic of China.
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MeSH Terms
Aged, 80 and over
Carcinoma, Hepatocellular / enzymology,  metabolism*,  pathology
Cell Cycle
Cell Cycle Proteins / metabolism*
G2 Phase / physiology
Hep G2 Cells
Intracellular Signaling Peptides and Proteins / metabolism*
JNK Mitogen-Activated Protein Kinases / metabolism*
Liver Neoplasms / enzymology,  metabolism*,  pathology
MAP Kinase Signaling System*
Middle Aged
Nuclear Proteins / metabolism*
p38 Mitogen-Activated Protein Kinases / metabolism*
Reg. No./Substance:
0/Cell Cycle Proteins; 0/GADD45A protein, human; 0/GADD45G protein, human; 0/Intracellular Signaling Peptides and Proteins; 0/Nuclear Proteins; EC Mitogen-Activated Protein Kinases; EC Mitogen-Activated Protein Kinases

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