| GaAlAs Laser Irradiation Induces Active Tertiary Dentin Formation after Pulpal Apoptosis and Cell Proliferation in Rat Molars. | |
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MedLine Citation:
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PMID: 21763899 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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INTRODUCTION: This study aimed to clarify pulpal responses to gallium-aluminum-arsenide (GaAlAs) laser irradiation. METHODS: Maxillary first molars of 8-week-old rats were irradiated at an output power of 0.5 or 1.5 W for 180 seconds, and the samples were collected at intervals of 0 to 14 days. The demineralized paraffin sections were processed for immunohistochemistry for heat-shock protein (HSP)-25 and nestin in addition to cell proliferation assay using bromodeoxyuridine (BrdU) labeling and apoptosis assay using deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL). RESULTS: Intense HSP-25 and nestin immunoreactivities in the odontoblast layer were weakened immediately after 0.5-W irradiation and recovered on day 1, resulting in slight tertiary dentin formation by day 14. On the contrary, 1.5-W irradiation immediately induced the loss of HSP-25 and nestin-immunoreactivities in the odontoblast layer. On day 1, numerous TUNEL-positive cells appeared in a degenerative zone that was surrounded by intense HSP-25 immunoreactivity. BrdU-positive cells occurred within the intensely HSP-25-immunopositive areas during days 2 through 5, whereas TUNEL-positive cells gradually decreased in number by day 5. HSP-25- and nestin-positive odontoblast-like cells were arranged along the pulp-dentin border by day 7, resulting in remarkable tertiary dentin formation on day 14. CONCLUSIONS: The output energy determined pulpal healing patterns after GaAlAs laser irradiation; the higher energy induced the apoptosis in the affected dental pulp including odontoblasts followed by active cell proliferation in the intense HSP-25-immunoreactive areas surrounding the degenerative tissue, resulting in abundant tertiary dentin formation. Thus, the optimal GaAlAs laser irradiation elicited intentional tertiary dentin formation in the dental pulp. |
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Authors:
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Yoshimi Shigetani; Natsuki Sasa; Hironobu Suzuki; Takashi Okiji; Hayato Ohshima |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of endodontics Volume: 37 ISSN: 1878-3554 ISO Abbreviation: J Endod Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7511484 Medline TA: J Endod Country: United States |
Other Details:
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Languages: eng Pagination: 1086-91 Citation Subset: D |
Copyright Information:
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Copyright © 2011 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Nigata, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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