Document Detail


GaAlAs Laser Irradiation Induces Active Tertiary Dentin Formation after Pulpal Apoptosis and Cell Proliferation in Rat Molars.
MedLine Citation:
PMID:  21763899     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
INTRODUCTION: This study aimed to clarify pulpal responses to gallium-aluminum-arsenide (GaAlAs) laser irradiation.
METHODS: Maxillary first molars of 8-week-old rats were irradiated at an output power of 0.5 or 1.5 W for 180 seconds, and the samples were collected at intervals of 0 to 14 days. The demineralized paraffin sections were processed for immunohistochemistry for heat-shock protein (HSP)-25 and nestin in addition to cell proliferation assay using bromodeoxyuridine (BrdU) labeling and apoptosis assay using deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL).
RESULTS: Intense HSP-25 and nestin immunoreactivities in the odontoblast layer were weakened immediately after 0.5-W irradiation and recovered on day 1, resulting in slight tertiary dentin formation by day 14. On the contrary, 1.5-W irradiation immediately induced the loss of HSP-25 and nestin-immunoreactivities in the odontoblast layer. On day 1, numerous TUNEL-positive cells appeared in a degenerative zone that was surrounded by intense HSP-25 immunoreactivity. BrdU-positive cells occurred within the intensely HSP-25-immunopositive areas during days 2 through 5, whereas TUNEL-positive cells gradually decreased in number by day 5. HSP-25- and nestin-positive odontoblast-like cells were arranged along the pulp-dentin border by day 7, resulting in remarkable tertiary dentin formation on day 14.
CONCLUSIONS: The output energy determined pulpal healing patterns after GaAlAs laser irradiation; the higher energy induced the apoptosis in the affected dental pulp including odontoblasts followed by active cell proliferation in the intense HSP-25-immunoreactive areas surrounding the degenerative tissue, resulting in abundant tertiary dentin formation. Thus, the optimal GaAlAs laser irradiation elicited intentional tertiary dentin formation in the dental pulp.
Authors:
Yoshimi Shigetani; Natsuki Sasa; Hironobu Suzuki; Takashi Okiji; Hayato Ohshima
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of endodontics     Volume:  37     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-07-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1086-91     Citation Subset:  D    
Copyright Information:
Copyright © 2011 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Affiliation:
Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Nigata, Japan.
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