Document Detail


GSTM1 polymorphism and styrene metabolism: insights from an acute accidental exposure.
MedLine Citation:
PMID:  12191879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two workers were accidentally exposed to unusually high styrene concentrations (>1000 ppm) for about 30 min. In addition to the main styrene metabolites, mandelic acid (MA) and phenylglyoxylic acid (PGA), other minor metabolites, including specific mercapturic acids, (R,R)- and (S,R)-N-acetyl-S-(1-phenyl-2-hydroxyethyl)-L-cysteine [(R,R)-M1 and (S,R)-M1] and (R,R)- and (S,R)-N-acetyl-S-(2-phenyl-2-hydroxyethyl)-L-cysteine [(R,R)-M2 and (S,R)-M2], 4-vinylphenol-glucuronide and -sulfate, and phenylglycine, were determined by Liquid Chromatography Electrospray Tandem Mass Spectrometry (LC-ESI-MS/MS) in urine samples collected 12, 24, 36, 48, 75 and 99 h after the episode. The genotypes of microsomal epoxide hydrolase, glutathione-S-transferases M1-1 (GSTM1), T1-1 (GSTT1) and P1-1 (GSTP1) were characterized by PCR-based methods. The two subjects showed similar peak levels of MA and PGA, as well as 4-vinylphenol conjugates, whereas mercapturic acids were five times higher in the subject bearing the GSTM1pos than in the GSTM1null subject. Also, relative proportions of diasteroisomers of mercapturic acids were influenced by the GSTM1 polymorphism.
Authors:
Paola Manini; Giuseppe De Palma; Paola Mozzoni; Roberta Andreoli; Diana Poli; Enrico Bergamaschi; Innocente Franchini; Antonio Mutti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicology letters     Volume:  134     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-22     Completed Date:  2002-10-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  201-8     Citation Subset:  IM    
Affiliation:
Laboratory of Industrial Toxicology, Department of Clinical Medicine, Nephrology and Health Sciences, University of Parma Medical School, via Gramsci 14, 43100 Parma, Italy. paola.manini@unipr.it
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MeSH Terms
Descriptor/Qualifier:
Accidents*
Adult
Chromatography, Liquid
DNA / analysis
Genotype
Glutathione Transferase / genetics*
Humans
Inhalation Exposure / analysis
Middle Aged
Occupational Exposure / analysis*
Polymerase Chain Reaction
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length
Spectrometry, Mass, Electrospray Ionization
Stereoisomerism
Styrene / pharmacokinetics*
Chemical
Reg. No./Substance:
100-42-5/Styrene; 9007-49-2/DNA; EC 2.5.1.18/Glutathione Transferase; EC 2.5.1.18/glutathione S-transferase M1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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