Document Detail


GRO alpha in the fetomaternal and amniotic fluid compartments during pregnancy and parturition.
MedLine Citation:
PMID:  8789556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PROBLEM: GRO alpha/MGSA is a new member of the chemokine superfamily CXC(alpha) and is produced by a variety of cells including macrophages, fibroblasts, epithelial, and endothelial cells, and keratinocytes. This chemokine has chemoattractant activity and may participate in neutrophil recruitment and activation during the course of intrauterine infection. This study was conducted to investigate the effect of labor and microbial invasion of the amniotic cavity (MIAC) on amniotic fluid, fetal, and maternal plasma GRO alpha concentrations. METHOD: A cross-sectional study was designed using parameters that included gestational age, results of amniotic fluid (AF) cultures, and labor status at the time of amniocentesis. Fluid was retrieved by transabdominal amniocentesis. MIAC was defined as a positive amniotic fluid culture for bacteria. Umbilical cord blood was retrieved at the time of delivery. Amniotic fluid, maternal and fetal plasma GRO alpha concentrations were measured with a sensitive and specific ELISA (Quantikine, R&D Systems, Minneapolis, MN). RESULTS: 1) GRO alpha was detectable in amniotic fluid, umbilical cord, and maternal plasma samples; 2) GRO alpha concentrations in amniotic fluid increased with advancing gestational age; 3) Both term and preterm gestations with MIAC were associated with higher amniotic fluid GRO alpha concentrations than those with sterile amniotic fluid, independent of the labor status (term, MIAC, labor: median 2.7 ng/ml, range 1.4-12.7 vs. term, no MIAC, labor: median 2.1 ng/ml, range 0.7-3.4, vs term, no MIAC, no labor: median 1.9 ng/ml, range 1.8-4.2; P < 0.005; preterm: MIAC median 5 ng/ml, range 0.6-47.9 vs. no MIAC: median 2.3 ng/ml, range 0.5-10; P < 0.008); 4) A strong correlation was found between umbilical cord plasma GRO alpha concentrations and neonatal neutrophil count, and between GRO alpha concentrations and white blood cell count in the amniotic fluid (r = 0.67, P < 0.0005 and r = 0.38, P < 0.001, respectively). CONCLUSION: GRO alpha is a physiologic constituent of amniotic fluid and cord blood. Amniotic fluid GRO alpha concentrations increase with gestational age. Intrauterine infection both preterm and at term is associated with an increase in GRO alpha concentrations of amniotic fluid, suggesting that GRO alpha may play an important role in recruitment of neutrophils into the amniotic cavity.
Authors:
J Cohen; F Ghezzi; R Romero; A Ghidini; M Mazor; J E Tolosa; L F Gonçalves; R Gomez
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  35     ISSN:  1046-7408     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-10-23     Completed Date:  1996-10-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  23-9     Citation Subset:  IM    
Affiliation:
Perinatology Research Branch, Intramural Division, National Institute of Child Health and Human Development, Bethesda, Maryland, USA.
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MeSH Terms
Descriptor/Qualifier:
Amniotic Fluid / chemistry,  immunology*
Chemokine CXCL1
Chemokines, CXC*
Chemotactic Factors / analysis*,  blood,  urine
Cross-Sectional Studies
Female
Fetal Blood / immunology
Growth Inhibitors / analysis
Growth Substances / analysis*,  blood,  urine
Humans
Intercellular Signaling Peptides and Proteins*
Labor, Obstetric / immunology*
Maternal-Fetal Exchange / immunology*
Pregnancy / immunology*
Pregnancy Complications, Infectious / immunology
Chemical
Reg. No./Substance:
0/CXCL1 protein, human; 0/Chemokine CXCL1; 0/Chemokines, CXC; 0/Chemotactic Factors; 0/Growth Inhibitors; 0/Growth Substances; 0/Intercellular Signaling Peptides and Proteins

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