| GPR119 regulates murine glucose homeostasis through incretin receptor-dependent and independent mechanisms. | |
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MedLine Citation:
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PMID: 21068156 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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G protein-coupled receptor 119 (GPR119) was originally identified as a β-cell receptor. However, GPR119 activation also promotes incretin secretion and enhances peptide YY action. We examined whether GPR119-dependent control of glucose homeostasis requires preservation of peptidergic pathways in vivo. Insulin secretion was assessed directly in islets, and glucoregulation was examined in wild-type (WT), single incretin receptor (IR) and dual IR knockout (DIRKO) mice. Experimental endpoints included plasma glucose, insulin, glucagon, glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic peptide (GIP), and peptide YY. Gastric emptying was assessed in WT, Glp1r-/-, DIRKO, Glp2r-/-, and GPR119-/- mice treated with the GPR119 agonist AR231453. AR231453 stimulated insulin secretion from WT and DIRKO islets in a glucose-dependent manner, improved glucose homeostasis, and augmented plasma levels of GLP-1, GIP, and insulin in WT and Gipr-/- mice. In contrast, although AR231453 increased levels of GLP-1, GIP, and insulin, it failed to lower glucose in Glp1r-/- and DIRKO mice. Furthermore, AR231453 did not improve ip glucose tolerance and had no effect on insulin action in WT and DIRKO mice. Acute GPR119 activation with AR231453 inhibited gastric emptying in Glp1r-/-, DIRKO, Glp2r-/-, and in WT mice independent of the Y2 receptor (Y2R); however, AR231453 did not control gastric emptying in GPR119-/- mice. Our findings demonstrate that GPR119 activation directly stimulates insulin secretion from islets in vitro, yet requires intact IR signaling and enteral glucose exposure for optimal control of glucose tolerance in vivo. In contrast, AR231453 inhibits gastric emptying independent of incretin, Y2R, or Glp2 receptors through GPR119-dependent pathways. Hence, GPR119 engages multiple complementary pathways for control of glucose homeostasis. |
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Authors:
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Grace Flock; Dianne Holland; Yutaka Seino; Daniel J Drucker |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-10 |
Journal Detail:
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Title: Endocrinology Volume: 152 ISSN: 1945-7170 ISO Abbreviation: Endocrinology Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-21 Completed Date: 2011-02-25 Revised Date: 2012-02-03 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 374-83 Citation Subset: AIM; IM |
Affiliation:
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Samuel Lunenfeld Research Institute Mt Sinai Hospital, 600 University Avenue TCP5-1004, Toronto, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Glucose / genetics, metabolism* Gastric Emptying / drug effects, genetics Gastric Inhibitory Polypeptide / blood Glucagon / blood Glucagon-Like Peptide 1 / blood Glucose Tolerance Test Insulin / blood Islets of Langerhans / drug effects, metabolism Male Mice Mice, Inbred C57BL Mice, Knockout Oxadiazoles / pharmacology Pyrimidines / pharmacology Receptors, G-Protein-Coupled / genetics, metabolism* Receptors, Gastrointestinal Hormone / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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14799//Canadian Institutes of Health Research; 82700//Canadian Institutes of Health Research; 82700//Canadian Institutes of Health Research; MOP-14799//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/AR 231453; 0/Blood Glucose; 0/Gpr119 protein, mouse; 0/Insulin; 0/Oxadiazoles; 0/Pyrimidines; 0/Receptors, G-Protein-Coupled; 0/Receptors, Gastrointestinal Hormone; 59392-49-3/Gastric Inhibitory Polypeptide; 89750-14-1/Glucagon-Like Peptide 1; 9007-92-5/Glucagon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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