Document Detail

The GPIIB-IIIa-like complex may function as a human melanoma cell adhesion receptor for thrombospondin.
MedLine Citation:
PMID:  2470606     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to determine whether a heterodimeric complex immunologically related to the fibrinogen receptor could function as a thrombospondin (TSP) receptor in TSP-mediated cell-substratum adhesion of human melanoma cells. We found that polyclonal antibodies to the platelet GPIIb-IIIa complex, GPIIIa, and the human vitronectin receptor inhibited TSP-mediated cell adhesion by 63-68%. Immunoprecipitation of detergent extracts of 125I-surface-labeled melanoma cells using either anti-human platelet GPIIb-IIIa or anti-human vitronectin receptor antibody revealed the presence of a single heterodimeric complex, suggesting that both antisera recognize the same integrin receptor, GPIIb-IIIa-like antigen. Adhesion of cells to TSP is likely mediated through a region of the TSP molecule containing the arginine-glycine-aspartic (RGD) peptide sequence, since cell attachment to TSP was inhibited 50-66% in the presence of peptides containing RGD. These results strongly suggest that a GPIIb-IIIa-like/vitronectin receptor can serve as a cell binding site for TSP in mediating cell-substratum adhesion.
G P Tuszynski; J Karczewski; L Smith; A Murphy; V L Rothman; K A Knudsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  182     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-07-12     Completed Date:  1989-07-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  473-81     Citation Subset:  IM    
Lankenau Medical Research Center, Philadelphia, Pennsylvania 19151.
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MeSH Terms
Antibodies, Monoclonal / diagnostic use
Antigens, CD36
Blood Platelets / immunology*
Cell Adhesion
Cell Line
Immune Sera
Immunoglobulin G
Melanoma / immunology*
Membrane Glycoproteins / metabolism*
Molecular Weight
Platelet Membrane Glycoproteins / isolation & purification,  metabolism*
Receptors, Mitogen / metabolism*
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD36; 0/Immune Sera; 0/Immunoglobulin G; 0/Membrane Glycoproteins; 0/Platelet Membrane Glycoproteins; 0/Receptors, Mitogen; 0/Thrombospondins

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