| The GPI anchor signal sequence dictates the folding and functionality of the Als5 adhesin from Candida albicans. | |
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MedLine Citation:
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PMID: 22509405 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Proteins destined to be Glycosylphosphatidylinositol (GPI) anchored are translocated into the ER lumen completely before the C-terminal GPI anchor attachment signal sequence (SS) is removed by the GPI-transamidase and replaced by a pre-formed GPI anchor precursor. Does the SS have a role in dictating the conformation and function of the protein as well? METHODOLOGY/PRINCIPAL FINDINGS: We generated two variants of the Als5 protein without and with the SS in order to address the above question. Using a combination of biochemical and biophysical techniques, we show that in the case of Als5, an adhesin of C. albicans, the C-terminal deletion of 20 amino acids (SS) results in a significant alteration in conformation and function of the mature protein. CONCLUSIONS/SIGNIFICANCE: We propose that the locking of the conformation of the precursor protein in an alternate conformation from that of the mature protein is one probable strategy employed by the cell to control the behaviour and function of proteins intended to be GPI anchored during their transit through the ER. |
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Authors:
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Mohammad Faiz Ahmad; Bhawna Yadav; Pravin Kumar; Amrita Puri; Mohit Mazumder; Anwar Ali; Samudrala Gourinath; Rohini Muthuswami; Sneha Sudha Komath |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-04-11 |
Journal Detail:
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Title: PloS one Volume: 7 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2012 |
Date Detail:
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Created Date: 2012-04-17 Completed Date: 2012-08-07 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e35305 Citation Subset: IM |
Affiliation:
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School of Life Sciences, Jawaharlal Nehru University, New Delhi, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Substitution Candida albicans Cell Adhesion Molecules / chemistry*, metabolism* Circular Dichroism Collagen Type IV / chemistry, metabolism Endoplasmic Reticulum / metabolism Escherichia coli / genetics Fungal Proteins / chemistry*, metabolism* Gene Expression Regulation Glycosylphosphatidylinositols / chemistry, genetics*, metabolism Protein Binding Protein Folding Protein Sorting Signals / genetics* Protein Structure, Secondary / genetics |
| Chemical | |
Reg. No./Substance:
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0/ALA1 protein, Candida albicans; 0/Cell Adhesion Molecules; 0/Collagen Type IV; 0/Fungal Proteins; 0/Glycosylphosphatidylinositols; 0/Protein Sorting Signals |
| Comments/Corrections | |
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