Document Detail

GMS is superior to PAS for diagnosis of onychomycosis.
MedLine Citation:
PMID:  18331569     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Onychomycosis is a common cause of deformed nails. Periodic acid-Schiff (PAS) stains are more sensitive than fungal cultures for diagnosing onychomycosis. We performed a retrospective study comparing the use of PAS and Gomori methenamine silver (GMS) stains for histopathologic diagnosis of onychomycosis. METHODS: GMS stains were performed on 20 PAS-positive and 51 PAS-negative cases from nail biopsies with a clinical diagnosis of onychomycosis. The PAS stained slides and GMS stained slides were evaluated for the presence of fungal hyphae. The results were analyzed with McNemar's test. RESULTS: All 20 PAS-positive cases were also positive with GMS stains. Of the 51 PAS-negative cases, GMS stains detected an additional five cases with fungal hyphae. GMS stains were quantitatively superior (p < 0.0253). GMS stains were also qualitatively superior. More fungal hyphae were highlighted and fungal hyphae were more easily recognized on low or medium power magnification. CONCLUSIONS: GMS stains are superior to PAS stains for the routine diagnosis of onychomycosis.
Zandra D'Hue; Susan M Perkins; Steven D Billings
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Publication Detail:
Type:  Journal Article     Date:  2008-03-10
Journal Detail:
Title:  Journal of cutaneous pathology     Volume:  35     ISSN:  1600-0560     ISO Abbreviation:  J. Cutan. Pathol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-08     Completed Date:  2008-12-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0425124     Medline TA:  J Cutan Pathol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  745-7     Citation Subset:  IM    
Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
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MeSH Terms
Diagnosis, Differential
Methenamine / chemistry*
Onychomycosis / pathology*
Periodic Acid-Schiff Reaction*
Retrospective Studies
Sensitivity and Specificity
Reg. No./Substance:

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