| GMS is superior to PAS for diagnosis of onychomycosis. | |
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MedLine Citation:
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PMID: 18331569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Onychomycosis is a common cause of deformed nails. Periodic acid-Schiff (PAS) stains are more sensitive than fungal cultures for diagnosing onychomycosis. We performed a retrospective study comparing the use of PAS and Gomori methenamine silver (GMS) stains for histopathologic diagnosis of onychomycosis. METHODS: GMS stains were performed on 20 PAS-positive and 51 PAS-negative cases from nail biopsies with a clinical diagnosis of onychomycosis. The PAS stained slides and GMS stained slides were evaluated for the presence of fungal hyphae. The results were analyzed with McNemar's test. RESULTS: All 20 PAS-positive cases were also positive with GMS stains. Of the 51 PAS-negative cases, GMS stains detected an additional five cases with fungal hyphae. GMS stains were quantitatively superior (p < 0.0253). GMS stains were also qualitatively superior. More fungal hyphae were highlighted and fungal hyphae were more easily recognized on low or medium power magnification. CONCLUSIONS: GMS stains are superior to PAS stains for the routine diagnosis of onychomycosis. |
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Authors:
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Zandra D'Hue; Susan M Perkins; Steven D Billings |
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Publication Detail:
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Type: Journal Article Date: 2008-03-10 |
Journal Detail:
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Title: Journal of cutaneous pathology Volume: 35 ISSN: 1600-0560 ISO Abbreviation: J. Cutan. Pathol. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-08 Completed Date: 2008-12-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0425124 Medline TA: J Cutan Pathol Country: Denmark |
Other Details:
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Languages: eng Pagination: 745-7 Citation Subset: IM |
Affiliation:
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Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Diagnosis, Differential Female Humans Male Methenamine / chemistry* Onychomycosis / pathology* Periodic Acid-Schiff Reaction* Retrospective Studies Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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100-97-0/Methenamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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