Document Detail


GLP-2 delays but does not prevent the onset of necrotizing enterocolitis in preterm pigs.
MedLine Citation:
PMID:  23343934     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Necrotizing enterocolitis (NEC) is complex disease thought to occur as a result of an immaturity of the gastrointestinal tract of preterm infants. Intestinal dysfunction induced by total parental nutrition (TPN) may increase the risk for NEC upon introduction of enteral feeding. We hypothesized that the intestinal trophic and anti-inflammatory actions previously ascribed to the gut hormone, glucagon-like peptide-2 (GLP-2), would reduce the incidence of NEC when given in combination with TPN in preterm piglets.
METHODS: Preterm, newborn piglets were nourished by TPN and infused continuously with either human GLP-2 (100 μg · kg⁻¹ · day⁻¹) or control saline for 2 days (n = 12/group). On day 3, TPN was discontinued and pigs were given orogastric formula feeding every 3 hours, and continued GLP-2 or control treatment until the onset of clinical signs of NEC for an additional 96 hours and tissue was collected for molecular and histological endpoints.
RESULTS: GLP-2 treatment delayed the onset of NEC but was unable to prevent a high NEC incidence (~70%) and severity that occurred in both groups. GLP-2-treated pigs had less histological injury and increased proximal intestinal weight and mucosal villus height, but not crypt depth or Ki-67-positive cells. Inflammatory markers of intestinal myeloperoxidase were unchanged and serum amyloid A levels were higher in GLP-2-treated pigs.
CONCLUSIONS: GLP-2 did not prevent NEC and a proinflammatory response despite some reduction in mucosal injury and increased trophic effect.
Authors:
Nancy M Benight; Barbara Stoll; Oluyinka O Olutoye; Jens J Holst; Douglas G Burrin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  56     ISSN:  1536-4801     ISO Abbreviation:  J. Pediatr. Gastroenterol. Nutr.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-29     Completed Date:  2014-01-10     Revised Date:  2014-04-07    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  623-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Inbred Strains
Animals, Newborn
Anti-Inflammatory Agents, Non-Steroidal / administration & dosage,  adverse effects,  metabolism,  therapeutic use*
Cesarean Section
Disease Models, Animal*
Enterocolitis, Necrotizing / etiology,  immunology,  physiopathology,  prevention & control*
Female
Glucagon-Like Peptide 2 / administration & dosage,  adverse effects,  metabolism,  therapeutic use*
Humans
Infusions, Parenteral
Intestinal Mucosa / drug effects*,  immunology,  metabolism,  ultrastructure
Intestine, Small / drug effects*,  immunology,  metabolism,  ultrastructure
Male
Microvilli / drug effects,  immunology,  metabolism,  ultrastructure
Parenteral Nutrition, Total / adverse effects
Pregnancy
Premature Birth / drug therapy*,  metabolism,  pathology,  physiopathology
Random Allocation
Serum Amyloid A Protein / analysis
Texas
Up-Regulation / drug effects
Grant Support
ID/Acronym/Agency:
P30 DK-56338/DK/NIDDK NIH HHS; P30 DK056338/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Glucagon-Like Peptide 2; 0/Serum Amyloid A Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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