| GLIS, a bioactive proteoglycan fraction from Ganoderma lucidum, displays anti-tumour activity by increasing both humoral and cellular immune response. | |
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MedLine Citation:
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PMID: 20888840 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: Ganoderma lucidum, a traditional Chinese medicine, is well known as a modulator of functions of the immune system as well as an anti-tumour agent. However, its active compounds and their molecular mechanisms of action are not well established. GLIS, a proteoglycan isolated from the fruiting body of G. lucidum, stimulates directly the activation of B lymphocytes. In this work, the immunoactivation capacities of GLIS as well as its anti-tumour effect were investigated in vitro and in vivo. MAIN METHODS: Tumour-bearing mice were prepared by inoculation of mouse sarcoma S180 cells into BALB/c mice. Lymphocytes and bone marrow-derived macrophages were isolated from spleen and tibia/femurs, respectively. After stimulation with GLIS different immune responses of these cells were analysed. Anti-tumour effect of GLIS was determined. KEY FINDINGS: After treatment with GLIS, spleen-derived B lymphocytes from tumour-bearing mice became activated, proliferated and produced large amounts of immunoglobulins. Bone marrow-derived macrophages from tumour-bearing mice also became activated after exposure to GLIS, and they produced important immunomodulatory substances, such as IL-1β, TNF-α and reactive nitrogen intermediates, like NO. GLIS markedly increased phagocytosis of macrophages, and very importantly, it markedly raised the macrophage-mediated tumour cytotoxicity. Treatment of mice with GLIS caused an inhibition of mouse sarcoma S180 tumour growth by 60% in vivo. SIGNIFICANCE: These results indicate that GLIS exhibits a capacity to increase remarkably both humoral and cellular immune activities of tumour-bearing mice and inhibits tumour growth significantly. The anti-tumour effect of GLIS results from its capacity to increase the host's immune activity. |
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Authors:
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Jingsong Zhang; Qingjiu Tang; Changyan Zhou; Wei Jia; Luis Da Silva; Long Duc Nguyen; Werner Reutter; Hua Fan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-01 |
Journal Detail:
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Title: Life sciences Volume: 87 ISSN: 1879-0631 ISO Abbreviation: Life Sci. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-05 Completed Date: 2010-12-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 628-37 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Institut für Biochemie und Molekularbiologie, CBF, Charité-Universitätsmedizin Berlin, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents / immunology, pharmacology* B-Lymphocytes / drug effects, immunology Fungal Proteins / immunology, pharmacology* Immunity, Cellular / drug effects* Immunity, Humoral / drug effects* Interleukin-1beta / drug effects, immunology Macrophages / drug effects, immunology Medicine, Chinese Traditional Mice Mice, Inbred BALB C Nitric Oxide / metabolism Proteoglycans / immunology, pharmacology* Sarcoma 180 / drug therapy*, immunology, pathology Tumor Necrosis Factor-alpha / drug effects, immunology |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Fungal Proteins; 0/GLIS protein, Ganoderma lucidum; 0/Interleukin-1beta; 0/Proteoglycans; 0/Tumor Necrosis Factor-alpha; 10102-43-9/Nitric Oxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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