| GLI1 is involved in cell cycle regulation and proliferation of NT2 embryonal carcinoma stem cells. | |
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MedLine Citation:
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PMID: 18241033 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hedgehog (HH) signaling plays a critical role during embryogenesis and regulates early development of multiple tissues and organs, including the central nervous system. Although much has been revealed of the diverse functions of the HH signaling pathway, it is still unclear how the effects of altered HH signaling are interpreted by specific cell types. We have investigated the role of the HH transcription factor glioma-associated oncogene homolog 1 (GLI1) in the human Ntera2/D1 (NT2) embryonal carcinoma stem cell line. The study revealed that expression of GLI1 and its direct transcriptional target Patched (PTCH) is downregulated in the early stages of retinoic acid-induced neuronal differentiation of NT2 cells. To identify transcriptional targets of the HH transcription factor GLI1 in NT2 cells, we performed global expression profiling following GLI1 RNA interference (RNAi). Of the 8500 transcripts represented on the microarrays, expression of 88 genes was downregulated and expression of 26 genes was upregulated. Nineteen of these genes are involved in cell cycle and proliferation. Further, GLI1 RNAi leads to a significant decrease in NT2 proliferation and changes expression of G1 phase cyclins. In conclusion, our results suggest that GLI1 is involved in cell cycle and proliferation control in the embryonal carcinoma stem cell line NT2. |
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Authors:
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Janni Vestergaard; Allan Lind-Thomsen; Mikkel W Pedersen; Hanne Ostergaard Jarmer; Mads Bak; Lis Hasholt; Niels Tommerup; Zeynep Tümer; Lars Allan Larsen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: DNA and cell biology Volume: 27 ISSN: 1044-5498 ISO Abbreviation: DNA Cell Biol. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-06-02 Completed Date: 2008-06-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9004522 Medline TA: DNA Cell Biol Country: United States |
Other Details:
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Languages: eng Pagination: 251-6 Citation Subset: IM |
Affiliation:
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Wilhelm Johannsen Centre for Functional Genome Research, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Differentiation Cell Proliferation* Embryonal Carcinoma Stem Cells / metabolism*, pathology G1 Phase / physiology* Gene Expression Profiling Gene Expression Regulation, Neoplastic Hedgehog Proteins / metabolism Humans Oligonucleotide Array Sequence Analysis RNA, Small Interfering / pharmacology Signal Transduction Transcription Factors / physiology* Transcription, Genetic Tretinoin / pharmacology Tumor Markers, Biological / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/GLI1 protein, human; 0/Hedgehog Proteins; 0/RNA, Small Interfering; 0/Transcription Factors; 0/Tumor Markers, Biological; 302-79-4/Tretinoin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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