| GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. | |
| | |
MedLine Citation:
|
PMID: 20666777 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
GI24, an immunoglobulin superfamily member, has been cloned from a placenta cDNA library as a gene product that promoted activation of matrix metalloproteinase (MMP)-2 mediated by membrane type (MT) 1-MMP. Co-expression of GI24 with MT1-MMP in HEK293T cells increased the cell-surface level of MT1-MMP concomitant with the cleavage of the GI24 at the juxtamembrane site to shed the extracellular domain. HT1080 fibrosarcoma cells stably transfected with the GI24 gene expressed a higher level of MT1-MMP and showed more invasive ability in collagen gel than the control cells. GI24 was cleaved in HT1080 cells, which was blocked by the administration of MMP inhibitor BB94 or transfection of small interfering RNA (siRNA) targeting MT1-MMP. GI24 expression is relatively high in some squamous carcinoma and hepatocarcinoma cell lines. Transfection of siRNA for GI24 into oral squamous carcinoma-derived HSC-4 cells, which express GI24 and MT1-MMP genes reduced the expression of not only GI24 but also MT1-MMP, and attenuated invasive growth in the collagen matrix. These results suggest that GI24 contributes to tumor-invasive growth in the collagen matrix by augmenting cell surface MT1-MMP. |
| | |
Authors:
|
Moustafa A Sakr; Takahisa Takino; Takahiro Domoto; Hiroshi Nakano; Richard W Wong; Motoko Sasaki; Yasuni Nakanuma; Hiroshi Sato |
Related Documents
:
|
1562737 - Inhibition of c-jun causes reversible proliferative arrest and withdrawal from the cell... 9003417 - Intracellular localization of the pde4a camp-specific phosphodiesterase splice variant ... 15458317 - Improvement of the thermoregulated t7 expression system by using the heat-sensitive laci. 8373417 - Antisense and sense cdna expression cloning using autonomously replicating vectors and ... 17186707 - Decreased internalisation of erbb1 mutants in lung cancer is linked with a mechanism co... 11426937 - Kinetics of efficient recombinant adeno-associated virus transduction in retinal pigmen... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Cancer science Volume: 101 ISSN: 1349-7006 ISO Abbreviation: Cancer Sci. Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-10-18 Completed Date: 2010-11-29 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101168776 Medline TA: Cancer Sci Country: England |
Other Details:
|
Languages: eng Pagination: 2368-74 Citation Subset: IM |
Copyright Information:
|
© 2010 Japanese Cancer Association. |
Affiliation:
|
Department of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Kanazawa, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blotting, Western Cell Line Cell Line, Tumor Cell Membrane / metabolism* Collagen / metabolism Enzyme Activation Female Gene Library Humans Matrix Metalloproteinase 14 / antagonists & inhibitors, genetics, metabolism* Matrix Metalloproteinase 2 / metabolism* Membrane Proteins / genetics, metabolism* Phenylalanine / analogs & derivatives, pharmacology Placenta / metabolism Pregnancy Protease Inhibitors / pharmacology RNA Interference Reverse Transcriptase Polymerase Chain Reaction Thiophenes / pharmacology Transfection |
| Chemical | |
Reg. No./Substance:
|
0/GI24 protein, human; 0/Membrane Proteins; 0/Protease Inhibitors; 0/Thiophenes; 130370-60-4/batimastat; 63-91-2/Phenylalanine; 9007-34-5/Collagen; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.80/Matrix Metalloproteinase 14 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Iron deficiency anaemia: an unusual presentation of lymphangioma.
Next Document: Hsa-mir-27a genetic variant contributes to gastric cancer susceptibility through affecting miR-27a a...