Document Detail

GH3B6 pituitary tumor cell proliferation is mediated by PKCalpha and PKCepsilon via ERK 1/2-dependent pathway.
MedLine Citation:
PMID:  20798497     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: In this report, we explored the role of PKCalpha and PKCe as mediators of phorbol 12-myristate13-acetate (PMA)-induced proliferation in pituitary tumor GH3B6 cells, and determined if the ERK1/2 and Akt pathways were activated.
METHODS: The GH3B6 cell proliferation was estimated by BrdU incorporation and the cell cycle progression by flow cytometric cell cycle analysis. We determined the expression of PKCalpha and PKCe in membrane and cytosolic fractions by western blotting. The subcellular redistribution of both PKC isozymes was analyzed by confocal microscopy.
RESULTS: Incubation with PMA for 15 min stimulated PKCalpha and PKCe activation, which was correlated with the phosphorylation of ERK1/2 but not Akt. The activation of both these PKC isozymes was closely associated with the stimulation of proliferation and the cell cycle progression induced by PMA in GH3B6 cells, an effect that was blocked by the inhibitors of PKCalpha (Gö6976) and PKCe (eV1-2). In addition, the pretreatment with the inhibitor of ERK1/2 (PD98059) prevented the mitogenic activity induced by treatment with PMA for 15 min.
CONCLUSION: We demonstrated that the activation of PKCalpha and PKCe by phorbol ester in tumor pituitary GH3B6 cells led to cell proliferation and cell cycle progression, effects that involved ERK1/2 activation.
Juan Pablo Petiti; Silvina Gutiérrez; Ana Lucía De Paul; Verónica Andreoli; Claudia Mariela Palmeri; Liliana Del Valle Sosa; José Luis Bocco; Alicia Inés Torres
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-24
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  26     ISSN:  1421-9778     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-27     Completed Date:  2010-12-08     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  135-46     Citation Subset:  IM    
Copyright Information:
Copyright 2010 S. Karger AG, Basel.
Centro de Microscopía Electrónica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
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MeSH Terms
Bromodeoxyuridine / pharmacology
Cell Proliferation
Flavonoids / pharmacology
Flow Cytometry
Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors,  metabolism*
Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors,  metabolism*
Pituitary Neoplasms / enzymology*
Protein Kinase C-alpha / antagonists & inhibitors,  metabolism*
Protein Kinase C-epsilon / antagonists & inhibitors,  metabolism*
Signal Transduction
Tetradecanoylphorbol Acetate / analogs & derivatives,  pharmacology
Tumor Cells, Cultured
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/Flavonoids; 16561-29-8/Tetradecanoylphorbol Acetate; 57716-89-9/4-O-methyl-12-O-tetradecanoylphorbol 13-acetate; 59-14-3/Bromodeoxyuridine; EC Kinase C-alpha; EC Kinase C-epsilon; EC Protein Kinase 1; EC Protein Kinase 3

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