Document Detail

GH3 cells expressing constitutively active Gs alpha (Q227L) show enhanced hormone secretion and proliferation.
MedLine Citation:
PMID:  9099899     Owner:  NLM     Status:  MEDLINE    
cAMP levels in GH3gsp cells (Q227L mutation of Gs alpha), in comparison with uninfected GH3 and GH3vt (with vector alone) cells, were two to three fold (P< 0.01) higher (basal), and 10-20 fold (P<0.001) higher (in the presence of isobutyl methylxanthine, (IBMX)). Proliferation of GH3gsp cells after 7 days in culture, as determined by cell number and [(3)H]thymidine incorporation, were up to 25% (respectively P <0.001 and P < 0.02) higher. After chronic (4 days) but not acute (15 min) exposure to forskolin (10 microM) or dibutyryl cAMP (50 microM) all cell types showed a greater than 200% (P < 0.001) increase in [(3)H]thymidine incorporation. Secretion of prolactin and growth hormone by GH3gsp cells were two to four fold (P <0.001) higher than GH3 and GH3vt cells after 4 h and 10-12 fold (P <0.001) higher after 8 h. In conclusion GH3 cells possessing Q227L have a higher proliferation rate and secrete higher levels of prolactin and growth hormone which are associated with higher levels of cAMP.
J Ham; M Ivan; D Wynford-Thomas; M F Scanlon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  127     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-06-17     Completed Date:  1997-06-17     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  41-7     Citation Subset:  IM    
Department of Medicine, University of Wales College of Medicine, Cardiff, UK. Jack Ham@CF.AC.UK
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MeSH Terms
1-Methyl-3-isobutylxanthine / pharmacology
Blotting, Western
Bucladesine / pharmacology
Cell Count
Cell Division / drug effects
Cyclic AMP / metabolism*
Enzyme Activation
Forskolin / pharmacology
GTP-Binding Proteins / genetics*
Growth Hormone / secretion*
Mutation / physiology*
Prolactin / secretion*
Thymidine / metabolism
Tumor Cells, Cultured / physiology
Reg. No./Substance:
28822-58-4/1-Methyl-3-isobutylxanthine; 362-74-3/Bucladesine; 50-89-5/Thymidine; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; 9002-62-4/Prolactin; 9002-72-6/Growth Hormone; EC 3.6.1.-/GTP-Binding Proteins

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