| GH overexpression modifies muscle expression of anti-oxidant enzymes and increases spinal curvature of old zebrafish. | |
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MedLine Citation:
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PMID: 20338232 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Growth hormone (GH) excess causes an increment in the metabolic rate and in reactive oxygen species generation, which accelerate the ageing process in mammals. Considering that there is no information on this subject in fish, the aim of the present study was to evaluate the excess GH effect on senescence in a zebrafish (Danio rerio) transgenic model. In order to reach this objective, we analyzed the phenotype of spinal curvature and expression of genes related to the anti-oxidant defense system and myogenesis in muscle of 8 and 30 months old GH-transgenic males. Gene expression analyses revealed that both superoxide dismutase isoforms were down-regulated only in 30 months old animals, while glutamate cysteine ligase was down-regulated in GH-transgenic zebrafish. Acceleration of the spinal curvature and a reduction in the expression of miogenin at both ages and MyoD in the old fish were also observed. Although neurolipofuscin accumulation was not significant in GH-transgenic zebrafish, the estimation of maximum longevity based on the von Bertalanffy growth function was significantly lower in this group. The results obtained here indicate that GH overexpression reduces the transcription of anti-oxidant defense system and myogenesis-related genes, which probably accelerates senescence in the zebrafish transgenic model used. |
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Authors:
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Carlos Eduardo da Rosa; Rafael Yutaka Kuradomi; Daniela Volcan Almeida; Carlos Frederico Ceccon Lannes; Márcio de Azevedo Figueiredo; Aline Guerra Dytz; Duane Barros Fonseca; Luis Fernando Marins |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-23 |
Journal Detail:
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Title: Experimental gerontology Volume: 45 ISSN: 1873-6815 ISO Abbreviation: Exp. Gerontol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-03 Completed Date: 2010-08-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: England |
Other Details:
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Languages: eng Pagination: 449-56 Citation Subset: IM |
Copyright Information:
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2010 Elsevier Inc. All rights reserved. |
Affiliation:
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PPGCF-FAC, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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physiology Animals Animals, Genetically Modified / genetics, metabolism Antioxidants / metabolism Carps / genetics Gene Amplification Gene Expression Regulation, Developmental Glutamate-Cysteine Ligase / genetics* Glutathione / genetics, metabolism Growth Charts Growth Hormone / genetics, metabolism*, physiology Lipid Peroxidation / genetics Lipofuscin Male Muscle Development / genetics* Muscle, Skeletal / metabolism Phenotype Polymerase Chain Reaction Protein Isoforms / genetics, metabolism RNA, Messenger / metabolism Spinal Curvatures / metabolism*, radiography Superoxide Dismutase / genetics* Zebrafish / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Lipofuscin; 0/Protein Isoforms; 0/RNA, Messenger; 70-18-8/Glutathione; 9002-72-6/Growth Hormone; EC 1.15.1.1/Superoxide Dismutase; EC 6.3.2.2/Glutamate-Cysteine Ligase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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