Document Detail

GFLV replication in electroporated grapevine protoplasts.
MedLine Citation:
PMID:  10814824     Owner:  NLM     Status:  Publisher    
Grapevine fanleaf virus (GFLV), responsible for the economically important court-noué disease, is exclusively transmitted to its natural host in the vineyards through Xiphinema nematodes. We have developed direct inoculation of GFLV into grapevine through protoplast electroporation. Protoplasts were isolated from mesophyll of in vitro-grown plants and from embryogenic cell suspensions. Permeation conditions were determined by monitoring calcein uptake. Low salt poration medium was selected. Electrical conditions leading to strong transient gene expression were also tested for GFLV inoculation (isolate F13). GFLV replication was detected with either virus particles (2 µg) or viral RNA (10 ng) in both protoplast populations, as shown by anti-P38 Western blotting. Direct inoculation and replication were also observed with Arabis mosaic virus (ArMV), a closely related nepovirus, as well as with another GFLV isolate. These results will be valuable in grapevine biotechnology, for GFLV replication studies, transgenic plant screening for GFLV resistance, and biorisk evaluation.
Valat; Toutain; Courtois; Gaire; Decout; Pinck; Mauro; Burrus
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Publication Detail:
Journal Detail:
Title:  Plant science (Shannon, Ireland)     Volume:  155     ISSN:  0168-9452     ISO Abbreviation:  Plant Sci.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-05-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882015     Medline TA:  Plant Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  203-212     Citation Subset:  -    
Laboratoire de Biologie et Physiologie Végétales (URVVC, UPRES EA 2069), URCA, BP1039, F-51687, Reims, France
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