Document Detail


GD1b-derived gangliosides modulate FcεRI endocytosis in mast cells.
MedLine Citation:
PMID:  21411813     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of the mast cell-specific gangliosides in the modulation of the endocytic pathway of FcεRI was investigated in RBL-2H3 cells and in the ganglioside-deficient cell lines, E5 and D1. MAb BC4, which binds to the α subunit of FcεRI, was used in the analysis of receptor internalization. After incubation with BC4-FITC for 30 min, endocytic vesicles in RBL-2H3 and E5 cells were dispersed in the cytoplasm. After 1 hr, the endocytic vesicles of the RBL-2H3 cells had fused and formed clusters, whereas in the E5 cells, the fusion was slower. In contrast, in D1 cells, the endocytic vesicles were smaller and remained close to the plasma membrane even after 3 hr of incubation. When incubated with BC4-FITC and subsequently imunolabeled for markers of various endocytic compartments, a defect in the endocytic pathway in the E5 and D1 cells became evident. In the D1 cells, this defect was observed at the initial steps of endocytosis. Therefore, the ganglioside derivatives from GD1b are important in the endocytosis of FcεRI in mast cells. Because gangliosides may play a role in mast cell-related disease processes, they provide an attractive target for drug therapy and diagnosis.
Authors:
Vivian Marino Mazucato; Adriana Maria Mariano Silveira E Souza; Liliana Martos Nicoletti; Maria Célia Jamur; Constance Oliver
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  59     ISSN:  1551-5044     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-17     Completed Date:  2011-05-06     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  428-40     Citation Subset:  IM    
Affiliation:
Department of Cell and Molecular Biology and Pathogenic Bioagents, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Endocytosis
Gangliosides / genetics,  physiology*
Mast Cells / physiology*
Mutation
Protein Transport
Rats
Receptors, IgE / physiology*
Chemical
Reg. No./Substance:
0/FcepsilonRI protein, rat; 0/Gangliosides; 0/Receptors, IgE; 19553-76-5/ganglioside, GD1b
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