| GC-MS determination of flavonoids and phenolic and benzoic acids in human plasma after consumption of cranberry juice. | |
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MedLine Citation:
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PMID: 14733499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A GC-MS method was developed for the determination of various flavonoids and phenolic and benzoic acids in human plasma. The procedure involved the extraction of flavonoids and phenolic and benzoic acids with ethyl acetate, followed by the derivatization of the phenolic and benzoic compounds with BSTFA (N,O-bis(trimethylsilyl) trifluoroacetamide) + TMCS (trimethylchlorosilane) reagent. The trimethylsilyl derivatives formed were separated and quantitated using GC-MS. Twenty flavonoids and phenolic and benzoic compounds have been well separated in the spiked human plasma without any interference. The average recovery was 79.3%. Several phenolic acids such as o-hydroxybenzoic, p-hydroxyphenylacetic, 2,3-dihydroxybenzoic, 2,4-dihydroxybenzoic, ferulic, sinapic, and benzoic acid were identified and quantified in human plasma after consumption of a cranberry juice. This developed method provides a simple, specific, and sensitive technique for the simultaneous determination of flavonoids and phenolic and benzoic acids in human plasma and is suitable for bioavailability and pharmacokinetic studies. |
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Authors:
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Kai Zhang; Yuegang Zuo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 52 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-01-21 Completed Date: 2004-03-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 222-7 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, Massachusetts 02747, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Benzoic Acids
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blood* Beverages* Flavonoids / blood* Fruit / chemistry Gas Chromatography-Mass Spectrometry / methods* Humans Phenols / blood* Vaccinium macrocarpon / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Benzoic Acids; 0/Flavonoids; 0/Phenols |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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