| GATEWAY technology and E. coli recombinant system produce a properly folded and functional recombinant allergen of the lipid transfer protein of apple (Mal d 3). | |
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MedLine Citation:
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PMID: 19778615 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lipid transfer protein of apple fruit, Mal d 3, has been produced as a soluble recombinant protein in transformed Escherichia coli cells using the GATEWAY technology. Circular dichroism spectra showing the protein essentially consists of alpha-helices indicate that the rMal d 3 is properly folded. The (1)H NMR spectra also indicates a correct fold for the recombinant allergen. The reactivity of rMal d 3 towards IgE from apple allergic patients and in vitro degranulation activity measured on transformed rat basophil leukemia cells expressing the human Fc epsilon RI alpha-subunit of rMal d 3 is similar to those of the native allergen purified from apple fruits. The expression of active rMal d 3 in E. coli is readily feasible and offers an interesting alternative to the production of recombinant allergen in the yeast Pichia pastoris. This expression in E. coli open the way to the modification of Mal d 3 by site-directed mutagenesis for immunotherapy purposes. |
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Authors:
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Jean-Philippe Borges; Rapha?l Culerrier; Didier Aldon; Annick Barre; Herv? Benoist; Olivier Saurel; Alain Milon; Alain Didier; Pierre Roug? |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-22 |
Journal Detail:
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Title: Protein expression and purification Volume: 70 ISSN: 1096-0279 ISO Abbreviation: Protein Expr. Purif. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-02-23 Completed Date: 2010-05-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9101496 Medline TA: Protein Expr Purif Country: United States |
Other Details:
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Languages: eng Pagination: 277-82 Citation Subset: IM |
Copyright Information:
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(c) 2009 Elsevier Inc. All rights reserved. |
Affiliation:
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Surfaces Cellulaires et Signalisation chez les V?g?taux, UMR UPS-CNRS 5546, Universit? de Toulouse, 24 Chemin de Borde Rouge, 31326 Castanet, Tolosan, France. joao.borges@fc.up.pt |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Allergens
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genetics* Animals Carrier Proteins / genetics* Cloning, Molecular Escherichia coli / metabolism Humans Malus / genetics* Rats Recombinant Proteins / chemistry, genetics |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Carrier Proteins; 0/Mal d 3 allergen; 0/Recombinant Proteins; 0/lipid transfer protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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