| Of the GATA-binding proteins, only GATA-4 selectively regulates the human IL-5 gene promoter in IL-5 producing cells which express multiple GATA-binding proteins. | |
MedLine Citation:
|
PMID: 9209438 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Interleukin-5 (IL-5) is produced by T lymphocytes and known to support B cell growth and eosinophilic differentiation of the progenitor cells. Using ATL-16T cells which express IL-5 mRNA, we have identified a region, within the human IL-5 gene promoter, that regulates IL-5 gene transcription. This cis-acting sequence contains the core binding motif, (A/T)GATA(A/G), for GATA-binding family proteins and thus suggests the involvement of these family members. In this report, we describe the cloning of human GATA-4 (hGATA-4) and show that hGATA-4 selectively interacts with the -70 GATA site within the IL-5 proximal promoter region. By promoter deletion and mutation analyses, we established this region as a positive regulatory element. Cotransfection experiments revealed that both hGATA-4 and PMA/A23187 stimulation are necessary for the IL-5 promoter activation. The requirement of another regulatory element called CLE0, which lies downstream of the -70 GATA site, was also demonstrated. ATL-16T cells express mRNA of three GATA-binding proteins, hGATA-2, hGATA-3 and hGATA-4, and each of them has a potential to bind to the consensus (A/T)GATA(G/ A) motif. However, using ATL-16T nuclear extract, we demonstrated that GATA-4 is the only GATA-binding protein that forms specific DNA-protein complex with the -70 GATA site. The electrophoretic mobility shift assay with extracts of COS cells expressing GATA-binding proteins showed that GATA-4 has the highest binding affinity to the -70 GATA site among the three GATA-binding proteins. When the transactivation ability was compared among the three, GATA-4 showed the highest activity. These results demonstrate the selective role of GATA-4 in the transcriptional regulation of the IL-5 gene in a circumstance where multiple members of the GATA-binding proteins are expressed. |
Authors:
|
T Yamagata; J Nishida; R Sakai; T Tanaka; Y Yazaki; H Hirai |
Related Documents
:
|
10224408 - Regulatory mechanisms of human t cell il-5 synthesis: differential roles of the proxima... 10989198 - Regulation of cd34 transcription by sp1 requires sites upstream and downstream of the t... 2837728 - An erythroid specific nuclear factor binding to the proximal caccc box of the beta-glob... 18327588 - Characterization of myr1, a dosage suppressor of ypt6 and ric1 deficient mutants. 6329208 - The effects of iodination on the receptor binding affinity of ovine prolactin. 9515058 - Role of individual n-linked glycosylation sites in the function and intracellular trans... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Leukemia Volume: 11 Suppl 3 ISSN: 0887-6924 ISO Abbreviation: Leukemia Publication Date: 1997 Apr |
Date Detail:
|
Created Date: 1997-08-07 Completed Date: 1997-08-07 Revised Date: 2013-03-04 |
Medline Journal Info:
|
Nlm Unique ID: 8704895 Medline TA: Leukemia Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 501-2 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Sequence Binding Sites Calcimycin / pharmacology Cell Line DNA-Binding Proteins / biosynthesis, metabolism* GATA2 Transcription Factor GATA3 Transcription Factor GATA4 Transcription Factor Humans Interleukin-5 / biosynthesis*, genetics Promoter Regions, Genetic* Recombinant Proteins / biosynthesis Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid T-Lymphocytes / immunology Tetradecanoylphorbol Acetate / pharmacology Trans-Activators / biosynthesis Transcription Factors / biosynthesis, metabolism* Transcription, Genetic* Transfection Zinc Fingers |
| Chemical | |
Reg. No./Substance:
|
0/DNA-Binding Proteins; 0/GATA2 Transcription Factor; 0/GATA2 protein, human; 0/GATA3 Transcription Factor; 0/GATA3 protein, human; 0/GATA4 Transcription Factor; 0/Interleukin-5; 0/Recombinant Proteins; 0/Trans-Activators; 0/Transcription Factors; 16561-29-8/Tetradecanoylphorbol Acetate; 52665-69-7/Calcimycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Development of erythroid cells from mouse embryonic stem cells in culture: potential use for erythro...
Next Document: Molecular mechanism of blastic crisis in chronic myelocytic leukemia.