Document Detail

GATA-2 and GATA-2/ER display opposing activities in the development and differentiation of blood progenitors.
MedLine Citation:
PMID:  12065419     Owner:  NLM     Status:  MEDLINE    
GATA-2 is a zinc finger transcription factor essential for the development of hematopoiesis. While GATA-2 is generally considered to play an important role in the biology of hematopoietic stem and progenitor cells, its function within these compartments is not well understood. Here we have employed both conditional expression of GATA-2 and conditional activation of a GATA-2/estrogen receptor (ER) chimera to examine the effect of enforced GATA-2 expression in the development and differentiation of hematopoietic progenitors from murine embryonic stem cells. Consistent with the phenotype of GATA-2 null animals, conditional expression of GATA-2 from a tetracycline-inducible promoter enhanced the production of hematopoietic progenitors. Conditional activation of a GATA-2/ER chimera produced essentially opposite effects to those observed with conditional GATA-2 expression. GATA-2 and GATA-2/ER differ in their binding activities and transcriptional interactions from other hematopoietic-associated transcription factors such as c-Myb and PU.1. While we have exploited these differences in activity to explore the transcriptional networks underlying hematopoietic cell fate determination, our results suggest that care should be taken in interpreting results obtained using only chimeric proteins.
Kenji Kitajima; Masaaki Masuhara; Takumi Era; Tariq Enver; Toru Nakano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  21     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-14     Completed Date:  2002-08-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  3060-9     Citation Subset:  IM    
Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, Suita 565-0871, Japan.
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MeSH Terms
Biological Markers
Cell Differentiation / physiology
Cell Line
DNA-Binding Proteins / genetics,  metabolism*
Erythropoietin / pharmacology
Estradiol / pharmacology
GATA2 Transcription Factor
Gene Expression Regulation, Developmental*
Genes, Reporter
Hematopoietic Stem Cells / drug effects,  physiology*
Mice, Transgenic
Protein Synthesis Inhibitors / pharmacology
Receptors, Estrogen / genetics,  metabolism*
Recombinant Fusion Proteins / genetics,  metabolism
Tetracycline / pharmacology
Thrombopoietin / pharmacology
Transcription Factors / genetics,  metabolism*
Transcription, Genetic
Zinc Fingers
Reg. No./Substance:
0/Biological Markers; 0/DNA-Binding Proteins; 0/GATA2 Transcription Factor; 0/GATA2 protein, human; 0/Gata2 protein, mouse; 0/Protein Synthesis Inhibitors; 0/Receptors, Estrogen; 0/Recombinant Fusion Proteins; 0/Transcription Factors; 11096-26-7/Erythropoietin; 50-28-2/Estradiol; 60-54-8/Tetracycline; 9014-42-0/Thrombopoietin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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