Document Detail

The GAMYB protein from barley interacts with the DOF transcription factor BPBF and activates endosperm-specific genes during seed development.
MedLine Citation:
PMID:  11846878     Owner:  NLM     Status:  MEDLINE    
HvGAMYB, a MYB transcription factor previously shown to be expressed in barley aleurone cells in response to gibberellin during germination, also has an important role in gene regulation during endosperm development. The mRNA was detected early (10 DAF) in the seeds where it accumulates, not only in the aleurone layer, starchy endosperm, nucellar projection and vascular tissue, but also in the immature embryo as shown by in situ hybridization analysis. The HvGAMYB protein, expressed in bacteria, binds to oligonucleotides containing the 5'-TAACAAC-3' or 5'-CAACTAAC-3' sequences, derived from the promoter regions of the endosperm-specific genes Hor2 and Itr1, encoding a B-hordein and trypsin-inhibitor BTI-CMe, respectively. Binding is prevented when these motifs are mutated to 5'-TgACAAg-3' and 5'-CgACTgAC-3'. Transient expression experiments in co-bombarded developing endosperms demonstrate that HvGAMYB trans-activates transcription from native Hor2 and Itr1 promoters through binding to the intact motifs described above. Trans-activation of the Hor2 promoter also requires an intact prolamine box (PB). This suggests that HvGAMYB interacts in developing barley endosperms with the PB-binding factor BPBF, an endosperm-specific DOF transcriptional activator of the Hor2 gene. The in vivo interaction experiment between HvGAMYB and BPBF was done in the yeast two-hybrid system, where HvGAMYB potentiates the BPBF trans-activation capacity through interaction with its C-terminal domain.
Isabel Diaz; Jesús Vicente-Carbajosa; Zamira Abraham; Manuel Martínez; Inés Isabel-La Moneda; Pilar Carbonero
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  29     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-15     Completed Date:  2002-06-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  453-64     Citation Subset:  IM    
Laboratorio de Bioquímica y Biología Molecular, Dpto. de Biotecnología-UPM, ETS I Agrónomos, 28040 Madrid, Spain.
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MeSH Terms
Base Sequence
Binding Sites
Blotting, Northern
DNA-Binding Proteins / genetics*,  metabolism
Gene Expression Regulation, Plant
Hordeum / genetics*,  growth & development
In Situ Hybridization
Plant Proteins / genetics*,  metabolism
Promoter Regions, Genetic / genetics
Protein Binding
Proto-Oncogene Proteins c-myb*
RNA, Messenger / genetics,  metabolism
Saccharomyces cerevisiae / genetics
Seeds / genetics,  growth & development
Transcription Factors / genetics*,  metabolism
Transcriptional Activation / genetics
Two-Hybrid System Techniques
Reg. No./Substance:
0/DNA-Binding Proteins; 0/ITR protein, Hordeum vulgare; 0/Plant Proteins; 0/Proto-Oncogene Proteins c-myb; 0/RNA, Messenger; 0/Transcription Factors; 8002-80-0/Glutens

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