Document Detail


GAL promoter-driven heterologous gene expression in Saccharomyces cerevisiae △gal8 strain at anaerobic alcoholic fermentation.
MedLine Citation:
PMID:  23043744     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The removal of Gal80 protein by gene disruption turned into efficient GAL promoter-driven heterologous gene expression under anaerobic alcoholic fermentation of Saccharomyces cerevisiae. By using lipase B from C. antarctica as a reporter, the relative strength of GAL10 promoter (P(GAL) (10) ) in Δgal80 mutant that does not require galactose as an inducer was compared to those of ADH1, PDC1, and PGK promoters which have been known to work well anaerobically in actively fermenting yeast cells under high glucose concentration. P(GAL) (10) in the Δgal80 mutant showed 0.8-fold (ADH1), 4-fold (PDC1), and 50-fold (PGK) in promoter strength. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Authors:
Jungoh Ahn; Kyung-Min Park; Hongweon Lee; Yeo-Jin Son; Eui-Sung Choi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-9
Journal Detail:
Title:  FEMS yeast research     Volume:  -     ISSN:  1567-1364     ISO Abbreviation:  FEMS Yeast Res.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101085384     Medline TA:  FEMS Yeast Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
Biotechnology Process Engineering Center, KRIBB, 111 Gwahangno, Yuseong-gu, Daejeon, Republic of Korea.
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