| GAL promoter-driven heterologous gene expression in Saccharomyces cerevisiae △gal8 strain at anaerobic alcoholic fermentation. | |
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MedLine Citation:
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PMID: 23043744 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The removal of Gal80 protein by gene disruption turned into efficient GAL promoter-driven heterologous gene expression under anaerobic alcoholic fermentation of Saccharomyces cerevisiae. By using lipase B from C. antarctica as a reporter, the relative strength of GAL10 promoter (P(GAL) (10) ) in Δgal80 mutant that does not require galactose as an inducer was compared to those of ADH1, PDC1, and PGK promoters which have been known to work well anaerobically in actively fermenting yeast cells under high glucose concentration. P(GAL) (10) in the Δgal80 mutant showed 0.8-fold (ADH1), 4-fold (PDC1), and 50-fold (PGK) in promoter strength. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
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Authors:
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Jungoh Ahn; Kyung-Min Park; Hongweon Lee; Yeo-Jin Son; Eui-Sung Choi |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-9 |
Journal Detail:
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Title: FEMS yeast research Volume: - ISSN: 1567-1364 ISO Abbreviation: FEMS Yeast Res. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101085384 Medline TA: FEMS Yeast Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Affiliation:
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Biotechnology Process Engineering Center, KRIBB, 111 Gwahangno, Yuseong-gu, Daejeon, Republic of Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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