Document Detail


GABAergic inputs from direct and indirect striatal projection neurons onto cholinergic interneurons in the primate putamen.
MedLine Citation:
PMID:  23296794     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Striatal cholinergic interneurons (ChIs) are involved in reward-dependent learning and the regulation of attention. The activity of these neurons is modulated by intrinsic and extrinsic γ-aminobutyric acid (GABA)ergic and glutamatergic afferents, but the source and relative prevalence of these diverse regulatory inputs remain to be characterized. To address this issue, we performed a quantitative ultrastructural analysis of the GABAergic and glutamatergic innervation of ChIs in the postcommissural putamen of rhesus monkeys. Postembedding immunogold localization of GABA combined with peroxidase immunostaining for choline acetyltransferase showed that 60% of all synaptic inputs to ChIs originate from GABAergic terminals, whereas 21% are from putatively glutamatergic terminals that establish asymmetric synapses, and 19% from other (non-GABAergic) sources of symmetric synapses. Double pre-embedding immunoelectron microscopy using substance P and Met-/Leu-enkephalin antibodies to label GABAergic terminals from collaterals of "direct" and "indirect" striatal projection neurons, respectively, revealed that 47% of the indirect pathway terminals and 36% of the direct pathway terminals target ChIs. Together, substance P- and enkephalin-positive terminals represent 24% of all synapses onto ChIs in the monkey putamen. These findings show that ChIs receive prominent GABAergic inputs from multiple origins, including a significant contingent from axon collaterals of direct and indirect pathway projection neurons.
Authors:
Kalynda Kari Gonzales; Jean-Francois Pare; Thomas Wichmann; Yoland Smith
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  521     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-06-05     Completed Date:  2014-01-13     Revised Date:  2014-04-14    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2502-22     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Choline O-Acetyltransferase / metabolism
Enkephalins / metabolism
Female
Immunoenzyme Techniques
Immunohistochemistry
Interneurons / metabolism*
Macaca mulatta
Microscopy, Electron
Neostriatum / cytology,  metabolism*
Neurons / metabolism*
Parasympathetic Nervous System / cytology,  physiology*
Putamen / cytology,  metabolism*
Substance P / metabolism
Tissue Fixation
gamma-Aminobutyric Acid / physiology*
Grant Support
ID/Acronym/Agency:
F31NS066754/NS/NINDS NIH HHS; P50NS071669/NS/NINDS NIH HHS; P51 OD011132/OD/NIH HHS; P51 RR000165/RR/NCRR NIH HHS; R01 NS037948/NS/NINDS NIH HHS; R01NS037948/NS/NINDS NIH HHS; R01NS062876/NS/NINDS NIH HHS; RR00165/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Enkephalins; 33507-63-0/Substance P; 56-12-2/gamma-Aminobutyric Acid; EC 2.3.1.6/Choline O-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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