| GABA release from cerebellar stellate cells is developmentally regulated by presynaptic GABA(B) receptors in a target-cell-specific manner. | |
| | |
MedLine Citation:
|
PMID: 19674089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Transmitter release from boutons along a common axon is often regulated depending on the postsynaptic target. Here, GABA release from cerebellar stellate cells onto Purkinje cells and other stellate cells was examined in acute cerebellar slices of 2- and 4-week-old mice. Consistent with previous findings on action potential-dependent GABA release, we found a developmental decrease in inhibitory inputs onto Purkinje cells but not onto stellate cells when recording miniature inhibitory postsynaptic currents (mIPSCs). Although amplitudes of mIPSCs were developmentally reduced in both cell types, mIPSC frequencies were decreased in Purkinje cells but were increased in stellate cells. Similarly, modulation of GABA release by presynaptic GABA(B) receptors changed during development in Purkinje cells but not in stellate cells, as demonstrated by the baclofen-mediated depression of mIPSC frequency and evoked IPSC (eIPSC) amplitudes. The selectively diminished baclofen effect in 4-week-old Purkinje cells correlated with a selective downregulation of presynaptic GABA(B) receptors at stellate cell-to-Purkinje cell synapses observed by immunoelectron microscopy analysis. Thus, expression of GABA(B) receptors in stellate cell axons and presynaptic modulation of GABA release appear to change during development in a target-cell-specific manner. |
| | |
Authors:
|
Simone Astori; Rafael Luján; Georg Köhr |
Related Documents
:
|
7242149 - An improved method for the bulk isolation of viable perikarya from postnatal cerebellum. 7123849 - Congenital "histiocytoid" cardiomyopathy: evidence suggesting a developmental disorder ... 9334269 - Meander tail acts intrinsic to granule cell precursors to disrupt cerebellar developmen... 6883129 - Further studies on the mitogenic response of cultured schwann cells to rat cns axolemma... 18490509 - Switching myelination on and off. 17445229 - Transplantation of conditionally immortal auditory neuroblasts to the auditory nerve. 12733269 - An immunohistochemical study of the gastrointestinal endocrine cells in the c57bl/6 mice. 11349169 - Differing mechanisms of inhibition of calcium increases in human uterine myocytes by in... 15261119 - Nicotinic modulation of area postrema neuronal excitability in rat brain slices. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-07 |
Journal Detail:
|
Title: The European journal of neuroscience Volume: 30 ISSN: 1460-9568 ISO Abbreviation: Eur. J. Neurosci. Publication Date: 2009 Aug |
Date Detail:
|
Created Date: 2009-08-27 Completed Date: 2009-11-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8918110 Medline TA: Eur J Neurosci Country: France |
Other Details:
|
Languages: eng Pagination: 551-9 Citation Subset: IM |
Affiliation:
|
Department of Molecular Neurobiology, Max-Planck-Institute for Medical Research, Heidelberg, Germany. simone.astori@unil.ch |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Age Factors Animals Cerebellum / cytology, metabolism, physiology* Electrophysiology Inhibitory Postsynaptic Potentials / physiology Mice Mice, Inbred C57BL Microscopy, Immunoelectron Miniature Postsynaptic Potentials / physiology Neural Inhibition / physiology Neurons / metabolism, physiology* Presynaptic Terminals / metabolism*, physiology Receptors, GABA-B / metabolism* Signal Processing, Computer-Assisted Synaptic Transmission / physiology Vesicular Inhibitory Amino Acid Transport Proteins / metabolism gamma-Aminobutyric Acid / secretion* |
| Chemical | |
Reg. No./Substance:
|
0/Receptors, GABA-B; 0/Vesicular Inhibitory Amino Acid Transport Proteins; 0/Viaat protein, mouse; 56-12-2/gamma-Aminobutyric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: 3D surface perception from motion involves a temporal-parietal network.
Next Document: Mechanisms of after-hyperpolarization following activation of fly visual motion-sensitive neurons.