Document Detail


GABA release from cerebellar stellate cells is developmentally regulated by presynaptic GABA(B) receptors in a target-cell-specific manner.
MedLine Citation:
PMID:  19674089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transmitter release from boutons along a common axon is often regulated depending on the postsynaptic target. Here, GABA release from cerebellar stellate cells onto Purkinje cells and other stellate cells was examined in acute cerebellar slices of 2- and 4-week-old mice. Consistent with previous findings on action potential-dependent GABA release, we found a developmental decrease in inhibitory inputs onto Purkinje cells but not onto stellate cells when recording miniature inhibitory postsynaptic currents (mIPSCs). Although amplitudes of mIPSCs were developmentally reduced in both cell types, mIPSC frequencies were decreased in Purkinje cells but were increased in stellate cells. Similarly, modulation of GABA release by presynaptic GABA(B) receptors changed during development in Purkinje cells but not in stellate cells, as demonstrated by the baclofen-mediated depression of mIPSC frequency and evoked IPSC (eIPSC) amplitudes. The selectively diminished baclofen effect in 4-week-old Purkinje cells correlated with a selective downregulation of presynaptic GABA(B) receptors at stellate cell-to-Purkinje cell synapses observed by immunoelectron microscopy analysis. Thus, expression of GABA(B) receptors in stellate cell axons and presynaptic modulation of GABA release appear to change during development in a target-cell-specific manner.
Authors:
Simone Astori; Rafael Luján; Georg Köhr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-07
Journal Detail:
Title:  The European journal of neuroscience     Volume:  30     ISSN:  1460-9568     ISO Abbreviation:  Eur. J. Neurosci.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-27     Completed Date:  2009-11-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8918110     Medline TA:  Eur J Neurosci     Country:  France    
Other Details:
Languages:  eng     Pagination:  551-9     Citation Subset:  IM    
Affiliation:
Department of Molecular Neurobiology, Max-Planck-Institute for Medical Research, Heidelberg, Germany. simone.astori@unil.ch
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Cerebellum / cytology,  metabolism,  physiology*
Electrophysiology
Inhibitory Postsynaptic Potentials / physiology
Mice
Mice, Inbred C57BL
Microscopy, Immunoelectron
Miniature Postsynaptic Potentials / physiology
Neural Inhibition / physiology
Neurons / metabolism,  physiology*
Presynaptic Terminals / metabolism*,  physiology
Receptors, GABA-B / metabolism*
Signal Processing, Computer-Assisted
Synaptic Transmission / physiology
Vesicular Inhibitory Amino Acid Transport Proteins / metabolism
gamma-Aminobutyric Acid / secretion*
Chemical
Reg. No./Substance:
0/Receptors, GABA-B; 0/Vesicular Inhibitory Amino Acid Transport Proteins; 0/Viaat protein, mouse; 56-12-2/gamma-Aminobutyric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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