Document Detail


GABA and glycine in synaptic vesicles: storage and transport characteristics.
MedLine Citation:
PMID:  1678614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
gamma-Aminobutyric acid (GABA) and glycine are major inhibitory neurotransmitters that are released from nerve terminals by exocytosis via synaptic vesicles. Here we report that synaptic vesicles immunoisolated from rat cerebral cortex contain high amounts of GABA in addition to glutamate. Synaptic vesicles from the rat medulla oblongata also contain glycine and exhibit a higher GABA and a lower glutamate concentration than cortical vesicles. No other amino acids were detected. In addition, the uptake activities of synaptic vesicles for GABA and glycine were compared. Both were very similar with respect to substrate affinity and specificity, bioenergetic properties, and regional distribution. We conclude that GABA, glycine, and glutamate are the only major amino acid neurotransmitters stored in synaptic vesicles and that GABA and glycine are transported by similar, if not identical, transporters.
Authors:
P M Burger; J Hell; E Mehl; C Krasel; F Lottspeich; R Jahn
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuron     Volume:  7     ISSN:  0896-6273     ISO Abbreviation:  Neuron     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1991-09-20     Completed Date:  1991-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809320     Medline TA:  Neuron     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  287-93     Citation Subset:  IM    
Affiliation:
Department of Neurochemistry, Max-Planck Institute for Psychiatry, Martinsried, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport / physiology
Cerebral Cortex / chemistry,  metabolism,  ultrastructure
Chromatography, High Pressure Liquid
Glutamates / analysis,  metabolism,  pharmacokinetics
Glutamic Acid
Glycine / analysis,  metabolism*,  pharmacokinetics
Medulla Oblongata / chemistry,  metabolism,  ultrastructure
Rats
Synaptic Vesicles / chemistry,  metabolism*,  physiology
gamma-Aminobutyric Acid / analysis,  metabolism*,  pharmacokinetics
Chemical
Reg. No./Substance:
0/Glutamates; 56-12-2/gamma-Aminobutyric Acid; 56-40-6/Glycine; 56-86-0/Glutamic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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