Document Detail


GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis.
MedLine Citation:
PMID:  11118940     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
gamma-Aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) have been shown to increase the reproduction of the Brachionus plicatilis (NH3L strain). In the present study, the endogenous presence of GABA and 5-HT in the rotifers B. plicatilis (NH3L and Kamiura strains) and Brachionus rotundiformis (Langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (HPLC). HPLC showed that GABA and 5-HT concentrations in the three rotifer strains range from 71 to 188 pmol/mg and from 12 to 64 pmol/mg, respectively. A total of 33 amino acids were also detected in B. plicatilis and B. rotundiformis, with glutamic acid, serine, glycine, taurine, threonine, alanine, arginine, proline, valine and isoleucine in high concentrations relative to other amino acids.
Authors:
W G Gallardo; A Hagiwara; K Hara; K Soyano; T W Snell
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part A, Molecular & integrative physiology     Volume:  127     ISSN:  1095-6433     ISO Abbreviation:  Comp. Biochem. Physiol., Part A Mol. Integr. Physiol.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2001-01-22     Completed Date:  2001-02-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9806096     Medline TA:  Comp Biochem Physiol A Mol Integr Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  301-7     Citation Subset:  IM    
Affiliation:
Graduate School of Marine Science and Engineering, Nagasaki University, Bunkyo 1-14, 852-8131, Nagasaki, Japan. gallardo@aqd.seafdec.org.ph
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Animals
Chromatography, High Pressure Liquid
Immunoassay
Serotonin / metabolism*
Spectrophotometry, Ultraviolet
gamma-Aminobutyric Acid / metabolism*
Chemical
Reg. No./Substance:
0/Amino Acids; 50-67-9/Serotonin; 56-12-2/gamma-Aminobutyric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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