Document Detail

G6PD is indispensable for erythropoiesis after the embryonic-adult hemoglobin switch.
MedLine Citation:
PMID:  15271799     Owner:  NLM     Status:  MEDLINE    
Glucose 6-phosphate dehydrogenase (G6PD) (EC is an essential enzyme for the rapid production of NADPH, as required on exposure to oxidative stress. Mouse embryonic stem (ES) cells can produce all embryonic and fetal/adult cell types. By studying the in vitro differentiation of embryoid bodies produced from G6pdDelta ES cells that are totally unable to produce G6PD protein, we found that these cells are able to differentiate into mesodermal cells, cardiomyocytes, hepatocytes, and primitive erythroid cells. However, we show here that, after the hemoglobin switch has taken place, definitive erythrocytes die by apoptosis. This apoptotic death is delayed by reducing agents and by a caspase inhibitor, but it is prevented only by the restoration of G6PD activity. Thus, G6PD proves indispensable for definitive erythropoiesis.
Francesca Paglialunga; Annalisa Fico; Ingram Iaccarino; Rosario Notaro; Lucio Luzzatto; Giuseppe Martini; Stefania Filosa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-07-22
Journal Detail:
Title:  Blood     Volume:  104     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-04     Completed Date:  2004-12-23     Revised Date:  2012-02-22    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3148-52     Citation Subset:  AIM; IM    
Istituto di Genetica e Biofisica Adriano Buzzati Traverso CNR, Via G. Marconi 12, 80125 Napoli, Italy.
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MeSH Terms
Biological Markers
Cell Differentiation / physiology
Cell Survival / physiology
Erythrocytes / cytology,  enzymology*
Erythropoiesis / physiology*
Fetal Hemoglobin / physiology
Gene Expression Regulation, Developmental
Glucosephosphate Dehydrogenase / genetics*,  metabolism*
Hemoglobins / physiology*
Hepatocytes / cytology
Mesoderm / cytology
Myocytes, Cardiac / cytology
Grant Support
Reg. No./Substance:
0/Biological Markers; 0/Hemoglobins; 9034-63-3/Fetal Hemoglobin; EC Dehydrogenase

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