| G6PD is indispensable for erythropoiesis after the embryonic-adult hemoglobin switch. | |
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MedLine Citation:
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PMID: 15271799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glucose 6-phosphate dehydrogenase (G6PD) (EC 1.1.1.42) is an essential enzyme for the rapid production of NADPH, as required on exposure to oxidative stress. Mouse embryonic stem (ES) cells can produce all embryonic and fetal/adult cell types. By studying the in vitro differentiation of embryoid bodies produced from G6pdDelta ES cells that are totally unable to produce G6PD protein, we found that these cells are able to differentiate into mesodermal cells, cardiomyocytes, hepatocytes, and primitive erythroid cells. However, we show here that, after the hemoglobin switch has taken place, definitive erythrocytes die by apoptosis. This apoptotic death is delayed by reducing agents and by a caspase inhibitor, but it is prevented only by the restoration of G6PD activity. Thus, G6PD proves indispensable for definitive erythropoiesis. |
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Authors:
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Francesca Paglialunga; Annalisa Fico; Ingram Iaccarino; Rosario Notaro; Lucio Luzzatto; Giuseppe Martini; Stefania Filosa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-07-22 |
Journal Detail:
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Title: Blood Volume: 104 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-11-04 Completed Date: 2004-12-23 Revised Date: 2012-02-22 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
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Languages: eng Pagination: 3148-52 Citation Subset: AIM; IM |
Affiliation:
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Istituto di Genetica e Biofisica Adriano Buzzati Traverso CNR, Via G. Marconi 12, 80125 Napoli, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Biological Markers Cell Differentiation / physiology Cell Survival / physiology Erythrocytes / cytology, enzymology* Erythropoiesis / physiology* Fetal Hemoglobin / physiology Gene Expression Regulation, Developmental Glucosephosphate Dehydrogenase / genetics*, metabolism* Hemoglobins / physiology* Hepatocytes / cytology Mesoderm / cytology Mice Myocytes, Cardiac / cytology Phenotype |
| Grant Support | |
ID/Acronym/Agency:
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324/BI//Telethon |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Hemoglobins; 9034-63-3/Fetal Hemoglobin; EC 1.1.1.49/Glucosephosphate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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