| G1-dependent prion protein expression in human glioblastoma cell line T98G. | |
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MedLine Citation:
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PMID: 12081137 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human glioblastoma cell line T98G produced a cellular form of prion protein (PrP(C)), and we confirmed expression of PrP mRNA by RT-PCR. Immunoblot analysis of whole cell lysate revealed one major (35 kDa) and two faint bands (31, 25 kDa) that reacted with monoclonal anti-human PrP antibody 3F4. Cells treated with tunicamycin produced only a 25 kDa band, representing a deglycosylated form of PrP. Similarly, peptide: N-glycosidase F treatment of whole cell lysate altered the Asn-linked form to the deglycosylated form. When T98G cells were cultured for a longer period, the amount of PrP(C) per cell increased on Day 4 to 16 in a time-dependent manner. When the cells were cultured at high cell-density, the cells on Day 4 produced the same amount of PrP(C) as those on Day 16 of the usual culture. Moreover, in a serum-free medium, cells cultured at a low cell-density produced the same amount of PrP(C) as those cultured at the high cell-density. These results demonstrate that PrP(C) production in T98G cells was dependent on the phase of the cell cycle, probably the G1 phase. |
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Authors:
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Yutaka Kikuchi; Tomoshi Kakeya; Takeshi Yamazaki; Kaori Takekida; Naoto Nakamura; Haruo Matsuda; Kosuke Takatori; Akio Tanimura; Ken-ichi Tanamoto; Jun-ichi Sawada |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 25 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-06-25 Completed Date: 2002-12-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 728-33 Citation Subset: IM |
Affiliation:
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Division of Microbiology, National Institute of Health Sciences, Tokyo, Japan. kikuchi@nihs.go.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Brain Neoplasms
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metabolism* Culture Media, Conditioned Culture Media, Serum-Free DNA / biosynthesis DNA Primers Glioblastoma / metabolism* Humans Immunoblotting Nuclear Proteins / metabolism* PrPC Proteins / biosynthesis* Prions / metabolism* RNA / biosynthesis, isolation & purification Reverse Transcriptase Polymerase Chain Reaction Time Factors Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Culture Media, Serum-Free; 0/DNA Primers; 0/Nuclear Proteins; 0/PRNP protein, human; 0/PrPC Proteins; 0/Prions; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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