Document Detail

The G1 cell cycle arrest of macrophages infected with Aggregatibacter actinomycetemcomitans.
MedLine Citation:
PMID:  20374514     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Infection of murine macrophage cell line J774.1 with the periodontopathic bacterium Aggregatibacter actinomycetemcomitans induces apoptotic cell death. The infection induces cell cycle arrest in the G1 phase prior to the appearance of apoptotic cells. This study determined the involvement of various cell cycle-related signal molecules in A. actinomycetemcomitans-induced G1 cell cycle arrest. MATERIALS AND METHODS: Cell cycle in J774.1 cells infected with A. actinomycetemcomitans was analyzed with a flow cytometer. Immunoblot analysis was also employed to determine the expression levels of intracellular signal molecules. RESULTS: Flow cytometric analysis revealed that the percentage of cells in the G1 phase increased to 77.2% at 12 h after A. actinomycetemcomitans infection. Additionally, according to immunoblot analysis, expression levels of hyperphosphorylated forms of retinoblastoma protein (ppRb) declined in J774.1 cells following A. actinomycetemcomitans infection, whereas hypophosphorylated Rb (pRb) expression levels were elevated slightly. Expression levels of cyclin D1 and D2 in the cells decreased gradually postinfection; CDK2, CDK4, CDK6 and cyclin E levels were not changed. Furthermore, postinfection, p21(CIP1/WAF1) expression increased at 6 h, followed by a subsequent decrease. CONCLUSION: These findings suggest that cyclin D1 and D2 and p21(CIP1/WAF1) participate in G1 cell cycle arrest in A. actinomycetemcomitans-infected J774.1 cells.
H Kasai; K Nakashima; M Yokota; T Nishihara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral diseases     Volume:  16     ISSN:  1601-0825     ISO Abbreviation:  Oral Dis     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-08     Completed Date:  2010-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508565     Medline TA:  Oral Dis     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  305-9     Citation Subset:  D    
Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental College, Kokurakita-ku, Kitakyushu, Japan.
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MeSH Terms
Actinobacillus actinomycetemcomitans / physiology*
Apoptosis / physiology*
Cell Line
Cyclin D1 / biosynthesis
Cyclin D2 / biosynthesis
Cyclin-Dependent Kinase Inhibitor p21 / biosynthesis
G1 Phase / physiology*
Macrophages / metabolism,  microbiology*
Retinoblastoma Protein / metabolism
Reg. No./Substance:
0/Cyclin D2; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Retinoblastoma Protein; 136601-57-5/Cyclin D1

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