Document Detail

G1-G2 aggrecan product that can be generated by M-calpain on truncation at Ala709-Ala710 is present abundantly in human articular cartilage.
MedLine Citation:
PMID:  17261541     Owner:  NLM     Status:  MEDLINE    
To elucidate the specific function of m-calpain in the metabolism of aggrecan in human articular cartilage, the prevalence and localization of a large glycosaminoglycan-bearing aggrecan product generated by m-calpain in human osteoarthritis (OA) cartilage were investigated. Extracts of human OA articular cartilage were analysed by immunostaining using new polyclonal anti-VPGVA antiserum that detects the COOH terminal neoepitope IVTQVVPGVA(709) generated by m-calpain-related cleavage within the keratan sulphate rich region of human aggrecan. Immunoblotting analyses of aggrecan populations in guanidine hydrochloride-extracts showed that OA cartilages contained anti-VPGVA positive aggrecan products with the COOH terminal neoepitope ... VPGVA(709), resulting from truncation between the Ala(709)-Ala(710) m-calpain-related cleavage site. This aggrecan product consisted of two NH(2) terminal globular domain (G1 and G2) and KS side chains. Immunohistochemical staining showed that anti-VPGVA positive staining was localized within chondrocytes and spread to the surrounding interterritorial matrix. Confocal microscopic analysis showed subcellular colocalization of anti-VPGVA and anti m-calpain. These results indicate that the aggrecan product with the COOH terminal neoepitope VPGVA(709) is synthesized regularly by intracellular processing in chondrocytes, and is present abundantly as a limited form of aggrecan. M-calpain is the major candidate of the proteinase to generate this aggrecan product during the intracellular aggrecan processing.
Hideaki Maehara; Kiichi Suzuki; Tomohiro Sasaki; Hidefumi Oshita; Eriko Wada; Toshiyuki Inoue; Katsuji Shimizu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-29
Journal Detail:
Title:  Journal of biochemistry     Volume:  141     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-19     Completed Date:  2007-11-28     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  469-77     Citation Subset:  IM    
Department of Orthopaedic Surgery, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, Japan.
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MeSH Terms
Aggrecans / metabolism*
Blotting, Western
Calpain / metabolism*
Cartilage, Articular / metabolism*
Chondrocytes / metabolism
Keratan Sulfate / metabolism
Osteoarthritis / metabolism
Reg. No./Substance:
0/Aggrecans; 0/Epitopes; 9056-36-4/Keratan Sulfate; EC 3.4.22.-/Calpain; EC 3.4.22.-/m-calpain

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