| G1-G2 aggrecan product that can be generated by M-calpain on truncation at Ala709-Ala710 is present abundantly in human articular cartilage. | |
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MedLine Citation:
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PMID: 17261541 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To elucidate the specific function of m-calpain in the metabolism of aggrecan in human articular cartilage, the prevalence and localization of a large glycosaminoglycan-bearing aggrecan product generated by m-calpain in human osteoarthritis (OA) cartilage were investigated. Extracts of human OA articular cartilage were analysed by immunostaining using new polyclonal anti-VPGVA antiserum that detects the COOH terminal neoepitope IVTQVVPGVA(709) generated by m-calpain-related cleavage within the keratan sulphate rich region of human aggrecan. Immunoblotting analyses of aggrecan populations in guanidine hydrochloride-extracts showed that OA cartilages contained anti-VPGVA positive aggrecan products with the COOH terminal neoepitope ... VPGVA(709), resulting from truncation between the Ala(709)-Ala(710) m-calpain-related cleavage site. This aggrecan product consisted of two NH(2) terminal globular domain (G1 and G2) and KS side chains. Immunohistochemical staining showed that anti-VPGVA positive staining was localized within chondrocytes and spread to the surrounding interterritorial matrix. Confocal microscopic analysis showed subcellular colocalization of anti-VPGVA and anti m-calpain. These results indicate that the aggrecan product with the COOH terminal neoepitope VPGVA(709) is synthesized regularly by intracellular processing in chondrocytes, and is present abundantly as a limited form of aggrecan. M-calpain is the major candidate of the proteinase to generate this aggrecan product during the intracellular aggrecan processing. |
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Authors:
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Hideaki Maehara; Kiichi Suzuki; Tomohiro Sasaki; Hidefumi Oshita; Eriko Wada; Toshiyuki Inoue; Katsuji Shimizu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-29 |
Journal Detail:
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Title: Journal of biochemistry Volume: 141 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-19 Completed Date: 2007-11-28 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 469-77 Citation Subset: IM |
Affiliation:
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Department of Orthopaedic Surgery, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aggrecans
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metabolism* Animals Blotting, Western Calpain / metabolism* Cartilage, Articular / metabolism* Chondrocytes / metabolism Epitopes Humans Keratan Sulfate / metabolism Osteoarthritis / metabolism Swine |
| Chemical | |
Reg. No./Substance:
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0/Aggrecans; 0/Epitopes; 9056-36-4/Keratan Sulfate; EC 3.4.22.-/Calpain; EC 3.4.22.-/m-calpain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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