Document Detail

G protein-coupled receptor 30 is an estrogen receptor in the plasma membrane.
MedLine Citation:
PMID:  16780796     Owner:  NLM     Status:  MEDLINE    
Recently, GPR30 was reported to be a novel estrogen receptor; however, its intracellular localization has remained controversial. To investigate the intracellular localization of GPR30 in vivo, we produced four kinds of polyclonal antibodies for distinct epitopes on GPR30. Immunocytochemical observations using anti-GPR30 antibody and anti-FLAG antibody show that FLAG-GPR30 localizes to the plasma membrane 24 h after transfection. Treatment with estrogen (17beta-estradiol or E2) causes an elevation in the intracellular Ca2+ concentration ([Ca2+]i) within 10 s in HeLa cells expressing FLAG-GPR30. In addition, E2 induces the translocation of GPR30 from the plasma membrane to the cytoplasm by 1 h after stimulation. Immunohistochemical analysis shows that GPR30 exists on the cell surface of CA2 pyramidal neuronal cells. The images on transmission electron microscopy show that GPR30 is localized to a particular region associated with the plasma membranes of the pyramidal cells. These data indicate that GPR30, a transmembrane receptor for estrogen, is localized to the plasma membrane of CA2 pyramidal neuronal cells of the hippocampus in rat brain.
Takeshi Funakoshi; Akie Yanai; Koh Shinoda; Michio M Kawano; Yoichi Mizukami
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-09
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  346     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-06-26     Completed Date:  2006-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  904-10     Citation Subset:  IM    
Center for Gene Research, Yamaguchi University, Yamaguchi 755-8505, Japan.
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MeSH Terms
Antibodies / immunology
Brain / metabolism,  ultrastructure
Cell Line
Cell Membrane / drug effects,  metabolism*
Cercopithecus aethiops
Estrogens / pharmacology
Microscopy, Electron, Transmission
Microscopy, Immunoelectron
Receptors, Estrogen / genetics,  immunology,  metabolism*
Receptors, G-Protein-Coupled / genetics,  immunology,  metabolism*
Reg. No./Substance:
0/Antibodies; 0/Estrogens; 0/Receptors, Estrogen; 0/Receptors, G-Protein-Coupled

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