Document Detail

G cells and gastrin in chronic alcohol-treated rats.
MedLine Citation:
PMID:  18249268     Owner:  NLM     Status:  MEDLINE    
Numerous reports have described gastric mucosal injury in rats treated with high ethanol concentrations. However, to the best of our knowledge, ultrastructural characteristics of G cells and antral gastrin levels have not been previously reported, either in rats that chronically consumed alcohol or in human alcoholics. The goal of this study was to examine the effect of ethanol consumption (8.5 g/kg) over a 4-month period, under controlled nutritional conditions, on antral and plasma levels of gastrin, ultrastructure of G cells, morphometric characteristics of G cells by stereological methods, and analysis of endocrine cells in the gastric mucosa by immunohistochemistry. The chronic alcohol consumption resulted in a nonsignificant decrease in gastrin plasma levels and unchanged antral gastrin concentrations. A slightly damaged glandular portion of the gastric mucosa and dilatation of small blood vessels detected by histological analysis, suggests that ethanol has a toxic effect on the mucosal surface. Chronic alcohol treatment significantly decreased the number of antral G cells per unit area, and increased their cellular, nuclear, and cytoplasmatic profile areas. In addition, the volume density and diameter of G-cell granules, predominantly the pale and lucent types, were increased, indicating inhibition of gastrin release. Ethanol treatment also decreased the number of gastric somatostatin-, serotonin-, and histamine-immunoreactive cells, except the somatostatin cells in the pyloric mucosa, as well as both G: D: enterochromaffin cells (EC) cell ratios in the antrum and D: ECL cell ratios in the fundus. These results indicate that the change of morphometric parameters in G cells may be related to cellular dysfunction. Our findings also suggest that regulation of G-cell secretion was not mediated by locally produced somatostatin in ethanol-consuming rats, but may involve gastric luminal content and/or neurotransmitters of gastric nerve fibers.
Vera Todorović; Vesna Koko; Mirela Budec; Mileva Mićić; Marjan Micev; Mirjana Pavlović; Sanja Vignjević; Neda Drndarević; Olivera Mitrović
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Alcohol (Fayetteville, N.Y.)     Volume:  42     ISSN:  0741-8329     ISO Abbreviation:  Alcohol     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-05     Completed Date:  2008-04-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502311     Medline TA:  Alcohol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37-45     Citation Subset:  IM    
Department of Experimental Pathology and Cytology, University of Belgrade - Institute for Medical Research, Dr Subotića 4, PO Box 102, 11129 Belgrade, Serbia.
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MeSH Terms
Ethanol / blood,  toxicity*
Gastrin-Secreting Cells / chemistry,  drug effects*,  pathology,  ultrastructure
Gastrins / analysis*,  blood
Rats, Wistar
Reg. No./Substance:
0/Gastrins; 64-17-5/Ethanol

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