Document Detail


G Protein-coupled cyclic AMP signaling in postmortem brain of subjects with mood disorders: effects of diagnosis, suicide, and treatment at the time of death.
MedLine Citation:
PMID:  10461903     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Components of cyclic AMP (cAMP) signaling were examined in postmortem cerebral cortex of a well characterized group of patients with mood disorders and nonpsychiatric control subjects. We measured G protein levels, adenylyl cyclase (AC) activity, and CREB levels in cerebral cortex of the subjects with respect to diagnosis, treatment, and suicide. There was no effect of diagnosis on any measure, except for a trend toward decreased stimulated AC activity in subjects with mood disorders relative to control subjects. We also detected a significant effect of suicide on temporal cortex CREB levels in subjects that died as a result of suicide relative to those that did not, which was more evident in patients with major depressive disorder. Bipolar disorder (BD) subjects treated with anticonvulsants at the time of death had decreased temporal cortex CREB levels relative to those not receiving anticonvulsants. Furthermore, we found a trend toward decreased occipital cortex G alpha(s) (short) levels in BD subjects treated with lithium. These results support the hypothesis of altered cAMP signaling in mood disorders and raise the possibility that factors other than diagnosis, such as treatment and suicide, may be relevant to cell-signaling abnormalities reported in the literature.
Authors:
D Dowlatshahi; G M MacQueen; J F Wang; J S Reiach; L T Young
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  73     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-09-14     Completed Date:  1999-09-14     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1121-6     Citation Subset:  IM    
Affiliation:
Mood Disorders Program, McMaster University, Hamilton, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / metabolism
Adult
Aged
Antidepressive Agents / pharmacology*,  therapeutic use
Bipolar Disorder / metabolism
Brain / drug effects,  enzymology,  metabolism*
Cyclic AMP / physiology*
Cyclic AMP Response Element-Binding Protein / metabolism
Depressive Disorder / metabolism
Female
Forskolin / pharmacology
GTP-Binding Protein alpha Subunits, Gi-Go / metabolism
GTP-Binding Protein alpha Subunits, Gs / metabolism
GTP-Binding Proteins / physiology*
Humans
Immunoblotting
Male
Middle Aged
Mood Disorders / diagnosis,  drug therapy,  metabolism*
Occipital Lobe / drug effects,  metabolism
Signal Transduction / physiology*
Suicide*
Temporal Lobe / drug effects,  metabolism
Chemical
Reg. No./Substance:
0/Antidepressive Agents; 0/Cyclic AMP Response Element-Binding Protein; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; EC 3.6.1.-/GTP-Binding Proteins; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, Gi-Go; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, Gs; EC 4.6.1.1/Adenylate Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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