Document Detail

G-CSF stabilizes cardiac electrophysiology and decreases infarct size during cardiac ischemic/reperfusion in swine.
MedLine Citation:
PMID:  21276206     Owner:  NLM     Status:  Publisher    
Aim:  Effects of granulocyte colony-stimulating factor (G-CSF) on cardiac electrophysiology during ischemic/reperfusion (I/R) period are unclear. We hypothesized that G-CSF stabilizes cardiac electrophysiology during I/R injury by prolonging the effective refractory period (ERP), increasing the ventricular fibrillation threshold (VFT) and decreasing the defibrillation threshold (DFT), and that the cardioprotection of G-CSF is via preventing cardiac mitochondrial dysfunction. Methods:  In intact heart protocol, pigs were infused with either G-CSF or vehicle (n=7 each group) without I/R induction. In I/R protocol, pigs were infused with G-CSF (0.33μg/kg/min) or vehicle (n=8 each group) for 30 minutes prior to a 45-minute left anterior descending artery occlusion and at reperfusion. Diastolic pacing threshold (DPT), ERP, VFT and DFT were determined in all pigs before and during I/R period. Rat's isolated cardiac mitochondria were used to test the protective effect of G-CSF (100 nM) in H(2) O(2) -induced mitochondrial oxidative damage. Results:  Neither G-CSF nor vehicle altered any parameter in intact-heart pigs. During ischemic period, G-CSF significantly increased the DPT, ERP and VFT without altering the DFT. During reperfusion, G-CSF continued to increase the DPT without altering other parameters. The infarct size was significantly decreased in the G-CSF group, compared to the vehicle. G-CSF could also prevent cardiac mitochondrial swelling, decrease ROS production, and prevent mitochondrial membrane depolarization. Conclusion:  G-CSF increases the DPT, ERP and VFT and reduces the infarct size, thus stabilizing the myocardial electrophysiology, and preventing fatal arrhythmia during I/R. The protective mechanism could be via its effect in preventing cardiac mitochondrial dysfunction.
Natnicha Kanlop; Savitree Thommasorn; Siripong Palee; Punate Weerateerangkul; Songkiet Suwansirikul; Siriporn Chattipakorn; Nipon Chattipakorn
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-29
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  -     ISSN:  1748-1716     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Scandinavian Physiological Society.
Cardiac Electrophysiology Unit, Department of Physiology Cardiac Electrophysiology Research and Training Center Department of Pathology, Faculty of Medicine Department of Oral Biology, Faculty of Dentistry Biomedical Engineering Center, Chiang Mai University, Chiang Mai, Thailand.
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