Document Detail

G-CSF promotes bone marrow cells to migrate into infarcted mice heart, and differentiate into cardiomyocytes.
MedLine Citation:
PMID:  15690975     Owner:  NLM     Status:  MEDLINE    
A recent study showed that granulocyte-colony stimulating factor (G-CSF) treatment improved the infarcted cardiac function. Although mobilized stem cells may affect it, the mechanism is unclear. In this study, we investigated the origins of stem cells and phenotypic changes of the migrated cells, and evaluated the efficacy of G-CSF. Eighteen C57BL/6 mice were irradiated (900 cGy) and GFP mouse-derived bone marrow cells (GFP-BMC: 10(6) cells) were injected via a tail vein followed by splenectomy 4 weeks later. Ligation of the left descending coronary artery was performed 2 weeks later. Recombinant human G-CSF (200 microg/kg/day) was injected for 3 days before and 5 days after ligation (group 1, n = 10). Saline was injected in group 2 (n = 8). Four weeks after infarction, hearts and other organs were fixed for histology. The survival rate after postoperative day 3 in group 1 was 100%, while that in group 2 was 50% (p = 0.03). Bone marrow-derived GFP cells (BMD-GFP) in group 1 (103.3+/-71.9/mm2) were located at the infarcted border area significantly more than those in group 2 (43.6+/-23.7/mm2) (p < 0.0001). BMD-GFP cells were positive for troponin I (16.6%), myosin heavy chain-slow (16.7%), and nestin (8.8%) in group 1. Ki-67-positive BMD-GFP in group 1 (10.0+/-7.0/mm2) were significantly more than those in group 2 (4.8+/-6.1/mm2) (p = 0.01). G-CSF increased the survival rate after infarction. G-CSF promoted BMC to migrate into the infarcted border area. Bone marrow was one of the origins of regenerated cardiomyocytes.
Shinya Fukuhara; Shinji Tomita; Takeshi Nakatani; Yoshinori Ohtsu; Michiko Ishida; Chikao Yutani; Soichiro Kitamura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell transplantation     Volume:  13     ISSN:  0963-6897     ISO Abbreviation:  Cell Transplant     Publication Date:  2004  
Date Detail:
Created Date:  2005-02-04     Completed Date:  2005-05-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  741-8     Citation Subset:  IM    
Department of Regenerative Medicine & Tissue Engineering, National Cardiovascular Center, Osaka, Japan.
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MeSH Terms
Bone Marrow Cells / drug effects*,  physiology
Bone Marrow Transplantation / methods*
Cell Differentiation / drug effects*,  physiology
Cell Movement / drug effects*,  physiology
Disease Models, Animal
Granulocyte Colony-Stimulating Factor / pharmacology*,  therapeutic use
Green Fluorescent Proteins
Intermediate Filament Proteins / metabolism
Ki-67 Antigen / metabolism
Mice, Inbred C57BL
Mice, Transgenic
Models, Biological
Myocardial Infarction / therapy*
Myocytes, Cardiac / drug effects,  physiology
Myosin Heavy Chains / metabolism
Nerve Tissue Proteins / metabolism
Radiation Chimera
Recombinant Fusion Proteins / pharmacology,  therapeutic use
Regeneration / drug effects,  physiology
Survival Rate
Transplantation Chimera
Treatment Outcome
Troponin I / metabolism
Reg. No./Substance:
0/Intermediate Filament Proteins; 0/Ki-67 Antigen; 0/Myosin Heavy Chains; 0/Nerve Tissue Proteins; 0/Recombinant Fusion Proteins; 0/Troponin I; 0/nestin; 143011-72-7/Granulocyte Colony-Stimulating Factor; 147336-22-9/Green Fluorescent Proteins

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