| G-CSF-mediated thrombopoietin release triggers neutrophil motility and mobilization from bone marrow via induction of Cxcr2 ligands. | |
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MedLine Citation:
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PMID: 21224471 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Emergency mobilization of neutrophil granulocytes (neutrophils) from the bone marrow (BM) is a key event of early cellular immunity. The hematopoietic cytokine granulocyte-colony stimulating factor (G-CSF) stimulates this process, but it is unknown how individual neutrophils respond in situ. We show by intravital 2-photon microscopy that a systemic dose of human clinical-grade G-CSF rapidly induces the motility and entry of neutrophils into blood vessels within the tibial BM of mice. Simultaneously, the neutrophil-attracting chemokine KC (Cxcl1) spikes in the blood. In mice lacking the KC receptor Cxcr2, G-CSF fails to mobilize neutrophils and antibody blockade of Cxcr2 inhibits the mobilization and induction of neutrophil motility in the BM. KC is expressed by megakaryocytes and endothelial cells in situ and is released in vitro by megakaryocytes isolated directly from BM. This production of KC is strongly increased by thrombopoietin (TPO). Systemic G-CSF rapidly induces the increased production of TPO in BM. Accordingly, a single injection of TPO mobilizes neutrophils with kinetics similar to G-CSF, and mice lacking the TPO receptor show impaired neutrophil mobilization after short-term G-CSF administration. Thus, a network of signaling molecules, chemokines, and cells controls neutrophil release from the BM, and their mobilization involves rapidly induced Cxcr2-mediated motility controlled by TPO as a pacemaker. |
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Authors:
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Anja Köhler; Katia De Filippo; Mike Hasenberg; Cindy van den Brandt; Emma Nye; Martin P Hosking; Thomas E Lane; Linda Männ; Richard M Ransohoff; Anja E Hauser; Oliver Winter; Burkhart Schraven; Hartmut Geiger; Nancy Hogg; Matthias Gunzer |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-01-11 |
Journal Detail:
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Title: Blood Volume: 117 ISSN: 1528-0020 ISO Abbreviation: Blood Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-22 Completed Date: 2011-07-13 Revised Date: 2012-04-23 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
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Languages: eng Pagination: 4349-57 Citation Subset: AIM; IM |
Affiliation:
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Institute of Molecular and Clinical Immunology, Otto-von-Guericke University, Magdeburg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow / immunology Bone Marrow Cells / cytology*, immunology Bone and Bones / cytology Cell Line Cell Movement Cells, Cultured Granulocyte Colony-Stimulating Factor / immunology* Humans Megakaryocytes / cytology, immunology Mice Mice, Inbred C57BL Neutrophils / cytology*, immunology Receptors, Interleukin-8B / immunology* Thrombopoietin / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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DK077762/DK/NIDDK NIH HHS; HL076604/HL/NHLBI NIH HHS; //Cancer Research UK |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Interleukin-8B; 143011-72-7/Granulocyte Colony-Stimulating Factor; 9014-42-0/Thrombopoietin |
| Comments/Corrections | |
Comment In:
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Blood. 2011 Apr 21;117(16):4166-7
[PMID:
21511963
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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