Document Detail


G-CSF increases the expression of VCAM-1 on stromal cells promoting the adhesion of CD34+ hematopoietic cells: studies under flow conditions.
MedLine Citation:
PMID:  15308328     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE AND METHODS: The knowledge of the mechanisms underlying the adhesive processes that lead to homing and/or mobilization of hematopoietic progenitor cells, and the influence of blood rheology, is still limited. We analyzed the impact of flow conditions on the adhesion of CD34+ peripheral blood progenitor cell (PBPC) to the adhesive proteins fibronectin, laminin, and collagen, and to stromal cells. RESULTS: Under static conditions, all the adhesive substrata assayed promoted adhesion of CD34+ PBPC, being higher on the stromal cells. Under flow conditions, adhesion of CD34+ PBPC was remarkable on stromal cells while insignificant onto the purified proteins. Exposure of stromal cell monolayers to granulocyte colony-stimulating factor (G-CSF) further enhanced PBPC adhesion. This effect correlated with the activation of p38 MAPK and with an increase in the expression of VCAM-1 on stromal cells exposed to G-CSF. In inhibitory assays, both an antibody to the G-CSFR and a specific inhibitor of the p38 MAPK blocked the effects induced by the cytokine. CONCLUSION: Our results provide direct evidence that in stromal cells G-CSF activates the signaling protein p38 MAPK, inducing expression of the adhesion receptor VCAM-1. This mechanism seems to promote adhesion of CD34+ cells on stromal cells and could play a potential role in homing events.
Authors:
Berta Fuste; Gines Escolar; Pedro Marin; Roberto Mazzara; Antonio Ordinas; Maribel Diaz-Ricart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental hematology     Volume:  32     ISSN:  0301-472X     ISO Abbreviation:  Exp. Hematol.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-08-13     Completed Date:  2004-09-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  765-72     Citation Subset:  IM    
Affiliation:
Servicio de Hemoterapia-Hemostasia, Hospital Clínic, IDIBAPS, Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD34 / analysis*
Cell Adhesion / drug effects*
Cells, Cultured
Granulocyte Colony-Stimulating Factor / pharmacology*
Hematopoietic Stem Cells / physiology*
Humans
Immunohistochemistry
Mitogen-Activated Protein Kinases / physiology
Stromal Cells / chemistry
Vascular Cell Adhesion Molecule-1 / analysis,  physiology*
p38 Mitogen-Activated Protein Kinases
Chemical
Reg. No./Substance:
0/Antigens, CD34; 0/Vascular Cell Adhesion Molecule-1; 143011-72-7/Granulocyte Colony-Stimulating Factor; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases

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