Document Detail


Fusion of the platelet-derived growth factor receptor beta to a novel gene CEV14 in acute myelogenous leukemia after clonal evolution.
MedLine Citation:
PMID:  9373237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromosomal translocations involving band 5q31-35 occur in several hematologic disorders. A clone with a t(5; 14)(q33; q32) translocation appeared at the relapse phase in a patient with acute myelogenous leukemia who exhibited a sole chromosomal translocation, t(7; 11), at initial diagnosis. After the appearance of this clone, the leukemia progressed with marked eosinophilia, and combination chemotherapy was ineffective. Southern blot analysis showed a rearrangement of the platelet-derived growth factor receptor beta (PDGFRbeta) gene at 5q33 which was not observed at initial diagnosis. This translocation resulted in a chimeric transcript fusing the PDGFRbeta gene on 5q33 with a novel gene, CEV14, located at 14q32. Expression of the 5' region of the PDGFRbeta cDNA, upstream of the breakpoint, was not detected. However, the 3' region of PDGFRbeta, which was transcribed as part of the CEV14-PDGFRbeta fusion gene, was detected. A partial cDNA for a novel gene, CEV14, includes a leucine zipper motif and putative thyroid hormone receptor interacting domain and is expressed in a wide range of tissues. The expression of a CEV14-PDGFRbeta fusion gene in association with aggressive leukemia progression suggests that this protein has oncogenic potential.
Authors:
A Abe; N Emi; M Tanimoto; H Terasaki; T Marunouchi; H Saito
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Blood     Volume:  90     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1997-12-23     Completed Date:  1997-12-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4271-7     Citation Subset:  AIM; IM    
Affiliation:
First Department of Internal Medicine, Nagoya University School of Medicine, Nagoya; Mitsubishi Bioclinical Laboratory Inc, Tokyo.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Chromosomes, Human, Pair 14*
Chromosomes, Human, Pair 5*
Clone Cells / chemistry
Eosinophilia / genetics,  pathology
Humans
In Situ Hybridization, Fluorescence
Leukemia, Myeloid, Acute / genetics*,  pathology
Molecular Sequence Data
Nuclear Proteins*
Oncogene Proteins, Fusion / chemistry,  genetics*
Platelet-Derived Growth Factor*
Polymerase Chain Reaction
Proteins / chemistry,  genetics*
Receptor, Platelet-Derived Growth Factor beta
Receptors, Platelet-Derived Growth Factor / genetics*
Translocation, Genetic*
Chemical
Reg. No./Substance:
0/CEV14-PDGFRbeta fusion protein, human; 0/Nuclear Proteins; 0/Oncogene Proteins, Fusion; 0/Platelet-Derived Growth Factor; 0/Proteins; 0/TRIP11 protein, human; EC 2.7.10.1/Receptor, Platelet-Derived Growth Factor beta; EC 2.7.10.1/Receptors, Platelet-Derived Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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