Document Detail

Fusion of negatively charged phospholipid vesicles by alpha-latrotoxin.
MedLine Citation:
PMID:  2365074     Owner:  NLM     Status:  MEDLINE    
alpha-Latrotoxin-induced fusion of liposomes has been described using large unilamellar vesicles composed of phosphatidylcholine/phosphatidylethanolamine/cardiolipin at a molar ratio of 2:3:5. Vesicle fusion was monitored by terbium/dipicolinic acid assay as well as by fluorescence energy transfer measurement. The enhancement of the fusogenic effect of LTX by low concentrations (0.1-3 mM) of CaCl2 has been demonstrated. The efficiency of other divalent cations on the LTX fusogenic activity was shown to decrease in the sequence Ca greater than Cd greater than Sr greater than Mg greater than Ba. LTX-induced fusion was accompanied by the increase of vesicle size measured by laser correlation spectroscopy. It is concluded that fusogenic action of LTX may be involved in its effect on synaptic apparatus.
V K Lishko; Terletskaya YaT; I O Trikash
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  266     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-08-14     Completed Date:  1990-08-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  99-101     Citation Subset:  IM    
Department of Neurochemistry, A.V. Palladin Institute of Biochemistry, Academy of Sciences, Ukrainian SSR, Kiev.
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MeSH Terms
Arthropod Venoms*
Cations, Divalent / pharmacology
Membrane Fusion*
Spider Venoms*
Reg. No./Substance:
0/Arthropod Venoms; 0/Cations, Divalent; 0/Liposomes; 0/Phospholipids; 0/Spider Venoms; 65988-34-3/alpha-latrotoxin; 7440-70-2/Calcium

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