Document Detail

Fusion of nearby inverted repeats by a replication-based mechanism leads to formation of dicentric and acentric chromosomes that cause genome instability in budding yeast.
MedLine Citation:
PMID:  20008936     Owner:  NLM     Status:  MEDLINE    
Large-scale changes (gross chromosomal rearrangements [GCRs]) are common in genomes, and are often associated with pathological disorders. We report here that a specific pair of nearby inverted repeats in budding yeast fuse to form a dicentric chromosome intermediate, which then rearranges to form a translocation and other GCRs. We next show that fusion of nearby inverted repeats is general; we found that many nearby inverted repeats that are present in the yeast genome also fuse, as does a pair of synthetically constructed inverted repeats. Fusion occurs between inverted repeats that are separated by several kilobases of DNA and share >20 base pairs of homology. Finally, we show that fusion of inverted repeats, surprisingly, does not require genes involved in double-strand break (DSB) repair or genes involved in other repeat recombination events. We therefore propose that fusion may occur by a DSB-independent, DNA replication-based mechanism (which we term "faulty template switching"). Fusion of nearby inverted repeats to form dicentrics may be a major cause of instability in yeast and in other organisms.
Andrew L Paek; Salma Kaochar; Hope Jones; Aly Elezaby; Lisa Shanks; Ted Weinert
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  23     ISSN:  1549-5477     ISO Abbreviation:  Genes Dev.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2009-12-31     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2861-75     Citation Subset:  IM    
Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA.
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MeSH Terms
Chromosomes, Fungal / genetics*
DNA Breaks
DNA Replication / genetics*
DNA, Fungal / genetics*
Genomic Instability*
Inverted Repeat Sequences / genetics*
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae Proteins / metabolism
Grant Support
Reg. No./Substance:
0/DNA, Fungal; 0/Saccharomyces cerevisiae Proteins
Comment In:
Genes Dev. 2010 Jan 1;24(1):5-9   [PMID:  20047996 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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