Document Detail

Fusion-mediated microinjection of lysozyme into HepG2 cells through hemagglutinin neuraminidase-depleted Sendai virus envelopes.
MedLine Citation:
PMID:  8294448     Owner:  NLM     Status:  MEDLINE    
The potential of reconstituted Sendai viral envelopes containing only the fusion protein (F-virosomes) was evaluated for a targeted cytosolic delivery of lysozyme to human hepatoblastoma cells (HepG2) in culture. 125I-Lysozyme loaded into F-virosomes was used to monitor its fusion-mediated transfer to the HepG2 cells. Using fusion assay based on the transfer of water soluble probe, we have demonstrated the existence of aqueous connection between F-virosomes and target cells. Target specificity of the F-virosomes was ensured by the strong interaction between terminal beta-galactose moiety of F protein and the asialoglycoprotein receptor on the membrane of HepG2 cells. Incubation of the loaded F-virosomes with cells resulted in fusion-mediated injection, as inferred from the ability of cells to internalize lysozyme in the presence of azide (an inhibitor of the endocytotic process). Binding as well as fusion of the F-virosomes to HepG2 cells was solely mediated by the F protein. Introduction of 125I-lysozyme into the HepG2 cells was confirmed by selective accumulation of acid and antibody-precipitable radioactivity in the cytosolic compartment. The structural integrity of the internalized lysozyme was also assessed. The potential usefulness of F-virosomes with defined specificities as biological carrier for both in vitro and in vivo cytosolic delivery of macromolecules and drugs has been established.
S Bagai; D P Sarkar
Related Documents :
11734208 - Targeting influenza virosomes to ovarian carcinoma cells.
15831748 - Unveiling the mechanisms of cell-cell fusion.
12773418 - Production of cloned pigs by whole-cell intracytoplasmic microinjection.
23827568 - Probing interaction of gram-positive and gram-negative bacterial cells with zno nanorods.
12414948 - Replication advantage and host factor-independent phenotypes attributable to a common n...
24076428 - Circumvention of resistance to photodynamic therapy in doxorubicin-resistant sarcoma by...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  269     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-02-25     Completed Date:  1994-02-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1966-72     Citation Subset:  IM    
Department of Biochemistry, University of Delhi South Campus, New Delhi, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Asialoglycoprotein Receptor
Asialoglycoproteins / metabolism
Biological Transport
Cell Line
Liver Neoplasms
Membrane Fusion
Muramidase / administration & dosage*,  metabolism*
Parainfluenza Virus 1, Human
Receptors, Cell Surface / metabolism*
Subcellular Fractions / metabolism
Tumor Cells, Cultured
Viral Fusion Proteins*
Reg. No./Substance:
0/Asialoglycoprotein Receptor; 0/Asialoglycoproteins; 0/Receptors, Cell Surface; 0/Viral Fusion Proteins; 26566-61-0/Galactose; EC; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The immunodominant 38-kDa lipoprotein antigen of Mycobacterium tuberculosis is a phosphate-binding p...
Next Document:  Melanoma growth stimulatory activity enhances the phosphorylation of the class II interleukin-8 rece...