Document Detail

Fusion-mediated microinjection of active amine and diamine oxidases into cultured cells: effect on protein and DNA synthesis in chick embryo fibroblasts and in glioma cells.
MedLine Citation:
PMID:  3032996     Owner:  NLM     Status:  MEDLINE    
Serum amine oxidase and/or porcine kidney diamine oxidase were trapped within reconstituted Sendai virus envelopes, and retained their activity. The trapped enzymes that were detected by radioimmunoblots were microinjected into cultured cells by fusion. When diamine oxidase was microinjected into cultured fibroblasts of chick or rat embryos, a temporary arrest in protein and DNA synthesis was observed. The inhibitory effect was more significant when both serum amine oxidase and kidney diamine oxidase were microinjected into those cultured cells. Fibroblasts of either chick or rat embryos transformed by Rous sarcoma virus were more susceptible to the injected enzymes than the normal cultures, showing a complete arrest in protein and DNA synthesis within 4 hours. Similar results were obtained by microinjecting diamine oxidase into cultured glioma cells. The injected enzyme catalyzed the oxidation of intracellular polyamines. The resulting oxidation product (hydrogen peroxide and aminoaldehydes) apparently caused the arrest in the synthesis of macromolecules.
U Bachrach; I Ash; L Abu-Elheiga; M Hershkovitz; A Loyter
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  131     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-06-11     Completed Date:  1987-06-11     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  92-8     Citation Subset:  IM    
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MeSH Terms
Amine Oxidase (Copper-Containing) / administration & dosage*,  pharmacology
Cell Fusion
Cell Transformation, Viral
Cells, Cultured
DNA, Viral / biosynthesis*
Electrophoresis, Polyacrylamide Gel
Fibroblasts / cytology,  drug effects*
Glioma / pathology*
Injections / methods
Oxidoreductases Acting on CH-NH Group Donors / administration & dosage*,  pharmacology
Parainfluenza Virus 1, Human
Polyamines / biosynthesis
Viral Envelope Proteins / metabolism
Reg. No./Substance:
0/DNA, Viral; 0/Polyamines; 0/Viral Envelope Proteins; 9004-70-0/Collodion; EC Oxidase (Copper-Containing); EC 1.5.-/Oxidoreductases Acting on CH-NH Group Donors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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