Document Detail

Fusion of dissociated fish embryonic cells.
MedLine Citation:
PMID:  915427     Owner:  NLM     Status:  MEDLINE    
The induction of a frequent fusion in dissociated fish embryonic cells is reported. Fusion was induced during mechanical isolation of blastomeres in normal saline solution or Ca2+-free saline solution by quickly bringing about physical contact between cells within about 1-5 min of dissociation. Fused blastomeres were obtained in about 18% yield from early morula cells in about 68% yield from early blastula cells and in about 5% yield from early gastrula cells.
S Mizukami; N Satoh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of embryology and experimental morphology     Volume:  40     ISSN:  0022-0752     ISO Abbreviation:  J Embryol Exp Morphol     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-12-29     Completed Date:  1977-12-29     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7906439     Medline TA:  J Embryol Exp Morphol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  265-70     Citation Subset:  IM    
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MeSH Terms
Blastocyst / cytology
Cell Fusion
Cell Separation
Fishes / embryology*
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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